Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . and belowa longitudinal line drawn through the center of its curvatures, while in the otherforms the ends lie on the projection of such a line. The organism moves in anoscillatory manner about its longitudinal axis, its movements being winding,bending and whipping, while in the spirilla the longitudinal axis remainsrigid. Schaudinn demonstrated the existence of a flagellum at each end, whilethe other spirochaete have an undulating membrane. 554 DIAGNOSTIC METHODS. These organisms are
Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . and belowa longitudinal line drawn through the center of its curvatures, while in the otherforms the ends lie on the projection of such a line. The organism moves in anoscillatory manner about its longitudinal axis, its movements being winding,bending and whipping, while in the spirilla the longitudinal axis remainsrigid. Schaudinn demonstrated the existence of a flagellum at each end, whilethe other spirochaete have an undulating membrane. 554 DIAGNOSTIC METHODS. These organisms are seen only with great difficulty in the specimens offresh blood, but thanks to the introduction of the ultra-condenser (the dark-field illuminator) we are in a position to see these organisms, both in the splenicand peripheral blood, although considerable practice is necessary to properlyadjust the light. These organisms do not take anihn dyes readily, so thatspecial methods have been advanced for their demonstration in smears. A verygood stain for them is the Goldhorn stain. The smears are fixed with pure. Fig. 148. -Spirochaete pallidEe and refringens. (Pitfield.)The darker ones are the refringens. methyl alcohol for 15 minutes and are then covered with the stain (polychromemethylene blue) for three to five seconds, when the excess is drained off. Thespecimens are then slowly introduced into clean water with the film sides the slide in this position for four to five seconds and then shake in thewater to remove the excess of the dye. The spirochsete appear of a violetcolor. This violet tint may be changed to a bluish-black by covering thespecimen with Grams iodin solution for 15 to 20 seconds, after which it iswashed and dried as usual and the examination made with the immersionlens. The writer has also found the use of the Giemsa stain very reliable,especially when the staining is continued for 18 hours (see Exudates). Otherstains, such as that of Levaditi, have been
Size: 1581px × 1581px
Photo credit: © The Reading Room / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1900, booksubjectdiagnosis, bookyear19
