. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. IV (b). IV 20 40 60 80 100 0 20 40 T 1 M E ( MINUTES; 60 80 100 FIGURE 4. Effect of cyanide and methylene blue on the respiration of oyster tissues, (a) gill; (b) mantle. I, control; II, 1(T3 M cyanide + 6 X 1(T5 M methylene blue; III, 10'3 M cyanide + 10"5 M methylene blue; IV, 10~3 M cyanide. Each manometric flask contained 50 mg. of gill pieces or 100 mg. of mantle pieces in ml. of sea water buffered with M glycine. Manometric measurements were started twenty minutes after the tissues were im- mersed in each
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. IV (b). IV 20 40 60 80 100 0 20 40 T 1 M E ( MINUTES; 60 80 100 FIGURE 4. Effect of cyanide and methylene blue on the respiration of oyster tissues, (a) gill; (b) mantle. I, control; II, 1(T3 M cyanide + 6 X 1(T5 M methylene blue; III, 10'3 M cyanide + 10"5 M methylene blue; IV, 10~3 M cyanide. Each manometric flask contained 50 mg. of gill pieces or 100 mg. of mantle pieces in ml. of sea water buffered with M glycine. Manometric measurements were started twenty minutes after the tissues were im- mersed in each medium containing the reagents. 4. Effect of cyanide on the respiration of oyster tissues The endogenous respiration of the oyster gill was depressed by M cyanide to 15-20^ of the control and the inhibition was partly restored to about 40% of the control in the presence of 6 X 10~5M methylene blue, or its nearly saturated solution in sea water; while a lower concentration of methylene blue, 1 X IQ~5M, was slightly effective to reverse the cyanide inhibition at an initial short period, but this effect completely disappeared within 40 to 60 minutes after the respiratory measurement was started (Fig. 4a). Very similar results, though the effect of 6 X 10"5M methylene blue was somewhat smaller, were also obtained in the cyanide inhibition of the oyster mantle respiration (Fig. 4b). DISCUSSION The marine lamellibranchs, Crassostrea gif/as, Pinctada niartensii and Mytilus crassitesta, used in this investigation have no oxygen carrier such as hemoglobin or hemocyanin in the blood. However, as reported here, their visceral cells possess a normal cytochrome system consisting of cytochromes a, b, c and o-3, , cytochrome oxidase. The absorption bands of cytochromes in their hearts being nearly equiva- lent to those in the cells of baker's yeast, it is inferred that the hearts probably contain as much cytochromes as yeast cells. Such high contents of cytochromes in the moll
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
