. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CELL MOTILITY 231 Reference: Biol. Bull. 193: 231-232. (October, Consumption of Oxygen by Isolated Skate Retinal Photoreceptors Robert Paul Malchow {University of Illinois at Chicago), Stephen C. Land1'3, Leena S. Patel2, and Peter J. S. Smith1 A major problem in understanding the regulation of cellular metabolism is the heterogeneity of tissue structure, not only with regard to the cellular composition, but also within the individual cells. An ingenious step was the development of a method for measuring the oxygen consumpti
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CELL MOTILITY 231 Reference: Biol. Bull. 193: 231-232. (October, Consumption of Oxygen by Isolated Skate Retinal Photoreceptors Robert Paul Malchow {University of Illinois at Chicago), Stephen C. Land1'3, Leena S. Patel2, and Peter J. S. Smith1 A major problem in understanding the regulation of cellular metabolism is the heterogeneity of tissue structure, not only with regard to the cellular composition, but also within the individual cells. An ingenious step was the development of a method for measuring the oxygen consumption of a single isolated cell, though without spatial resolution around the cell itself (I). In this study, a solitary, amphibian rod photorecep- tor was used. These cells come from the retina, a tissue with an especially high metabolic rate (2), reflecting primarily the contribution of the photoreceptors (3). Previous measure- ments of retinal oxygen consumption have suggested that the metabolic activity of the vertebrate retina varies with light intensity (4), but neuromodulators, such as dopamine, may also substantially alter the metabolic activity of retinal neu- rons (5, 6). With one exception (1), these studies have been conducted using whole retinas, composed of a variety of cell types. Consequently, it has been difficult to determine clearly the oxygen consumption of different categories of cell. Oxy- gen-consumption experiments performed on isolated, identi- fiable retinal cells, with good spatial resolution, would provide new information on the control and modulation of metabolic activity in photoreceptors and provide an approach applicable to numerous other varieties of cells. The purpose of the present study was to measure oxygen consumption by isolated photoreceptors where there is a high degree of structural polarization, with improved spatial resolu- tion. This involved the use of a new technique incorporating polarographic oxygen-selective microelectrodes in a se
Size: 1793px × 1393px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
