. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 162 T. R. LACOURSE AND R. B. NORTHROP. FIGURE 1. Isolation of the ABRM and its associated nerve, the cerehropedal connective nerve (CPC), from the nervous system. Bars represent the points at which the nerve was severed; and an X shows the point of hooking by an extracellular electrode. cerebropedal connective (CPC) nerve, part of the foot, part of the cerebrovisceral connective nerve, and the visceral nerves. At this point it was important to assess that the animals were in good condi- tion. The criteria were that the mantl
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 162 T. R. LACOURSE AND R. B. NORTHROP. FIGURE 1. Isolation of the ABRM and its associated nerve, the cerehropedal connective nerve (CPC), from the nervous system. Bars represent the points at which the nerve was severed; and an X shows the point of hooking by an extracellular electrode. cerebropedal connective (CPC) nerve, part of the foot, part of the cerebrovisceral connective nerve, and the visceral nerves. At this point it was important to assess that the animals were in good condi- tion. The criteria were that the mantle should not tear awav from the shell upon light teasing of the tisstie, and the foot, on being mechanically stimulated, should be sharply retracted. If these conditions were not met the animal was eliminated from the study. Any mussels with visible bacterial, fungal or nematode infestations were also not used. By severing selected nerves at indentifiable points in the nervous svstem, the left ABRM and its innervating visceral nerves arising from the CPC were isolated from the rest of the nervous system (see Fig. 1 ). According to Twarog's (1960a) study, the innervation of the ABRMs comes entirely from the cerebral-pedal con- nectives, between the pedal ganglia and the branching of the cerebrovisceral con- nectives through the visceral nerves. To measure muscle tension, a nonelastic cord was attached to a crop of byssus threads by surgical silk at one-half the length of the byssus threads. The other end of the nonelastic cord was attached to a force/displacement transducer. Recording from the CPC nerve was accomplished by hooking the nerve with a platinum hook electrode insulated down to the booked tip with GC Electronics Liquid-Tape. Drying of the CPC nerve was prevented by enveloping the hooked CPC nerve recording site with vasoline diluted to a workable consistency with parafm oil. The reference electrode, constructed the same way as the recording electrode except the bare t
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
