. Animal tissue techniques. Histology -- Technique; Stains and staining (Microscopy); Histochemistry -- Technique. Sfainirig Procedures 115 decolorizino, and other solutions can result in a poorly stained slide. Hematoxylin-eosin staining (page 130) provides a simple example. If the eosin (counterstain) is applied before the hematoxylin (nuclear) stain, the former stain will be completely removed during the action of the latter stain. If slides are to be transported in quantity, rather than individually, several types of holders are useful and are on the market. Thomas has one holding 50
. Animal tissue techniques. Histology -- Technique; Stains and staining (Microscopy); Histochemistry -- Technique. Sfainirig Procedures 115 decolorizino, and other solutions can result in a poorly stained slide. Hematoxylin-eosin staining (page 130) provides a simple example. If the eosin (counterstain) is applied before the hematoxylin (nuclear) stain, the former stain will be completely removed during the action of the latter stain. If slides are to be transported in quantity, rather than individually, several types of holders are useful and are on the market. Thomas has one holding 50 slides; Wards has one for six slides. Some are baskets, others are clips, and fit into special staining dishes. The tissue-processing machines are equipped with slide carriers to fit the instrument. Phos- phor-bronze spring wire, inch di- ameter, can be fashioned into coils of I inch diameter and cut into any length to hold any^vhere from 3 or 4 slides up to 15 or 20. (Fig. 23) This combination of slides and coil can be used in rectangu- lar staining dishes with the slides resting on their long edges, or standing upright in tall stender dishes. A final processing of slides is necessary to make permanent preparations for ex- amination and storage without deteriora- tion. All alcohol and water must be extracted (with certain exceptions) and a medium applied which maintains the tissues in a clear and transparent condi- tion, does not alter the color or intensity of the stains, and holds a cover glass in place. The water is removed through increasing concentration of alco- hol until absolute alcohol is reached. The final reagent is xylene (or the like) to remove the alcohol and make the sections lose their opacity and thereby become clear. Finally a mounting medium (mountant) is applied and the cover glass lowered into place completely covering the sections. (The solvent of the mounting medium usually is either toluene or xylene.). Figure 23. A staining coil carrying multiple
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