. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. •>:- . :*r-. Figure I. Whole-mount preparations of the hindbrains of 2-cm frog tadpoles viewed from the ventral surface. (A) Brightfie/d photomicro- graph showing the rhombomeric segments (rhs 1—8) as a series of dark and light stripes, (B) Combined brightfield-epifluorescence overlay of a hindbrain after labeling of the Vlth nerve on the right side and the Vllth nerve on the left side with rhodamine-dextran. Facial motoneurons (fact reside in rhs 4 onlv, while abducens (aba) and accessory abducens (acc abd) reside in rhs
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. •>:- . :*r-. Figure I. Whole-mount preparations of the hindbrains of 2-cm frog tadpoles viewed from the ventral surface. (A) Brightfie/d photomicro- graph showing the rhombomeric segments (rhs 1—8) as a series of dark and light stripes, (B) Combined brightfield-epifluorescence overlay of a hindbrain after labeling of the Vlth nerve on the right side and the Vllth nerve on the left side with rhodamine-dextran. Facial motoneurons (fact reside in rhs 4 onlv, while abducens (aba) and accessory abducens (acc abd) reside in rhs 5 only. (C) Location of the trochlear (tro). trigeminal {tri), facial (fac). abducens (abd) and glossopharyngeal (glo), vagal (vag), accessory (acc) motor neurons as imaged in confocal preparations superimposed on the brightfield micrograph shown in A. Calibration bar in C applies to A and B as well. 24-26 larval frogs (Rana clamitans) were reconstructed with confocal microscopy after the cranial nerves were labeled with fluorescent tracers. The locations of the labeled neurons were correlated with rhombomeric compartments imaged in the same preparations by using oblique transmitted illumination. Crystals of 3000 mw dextran-conjugated rhodamine, fluorescein, or Texas red (Molecular Probes) were placed on the cut ends of the IVth, Vth. Vlth. Vllth. and IX-XIth cranial nerves. After transport times ranging from 24 to 48 h, the samples were fixed in 4% paraformaldehyde. Whole brains were cleared in glycerol and were either scanned on a Zeiss LSM-510 confocal micro- scope at 4-7 fjim z-axis intervals or photographed with transmit- ted and epi-illumination with a Spot digital camera on a Zeiss Axiophot microscope. The larval frog hindbrain appears as a rostro-caudal series of eight darkly pigmented regions of similar size, separated by narrower, more lightly pigmented stripes (Fig. 1 A). In the youn- gest tadpoles studied, an undulating pattern of sulci and gyri was superimpose
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
