. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 214 P. J. McAULEY Treatment of freshly isolated symbionts at pH 5 caused a significant reduction in both glutamate and glutamine pools compared to those at pH 7 (one-way ANOVA, P <). Incubation of intact green hydra in MSX caused no significant change in the glutamate or glutamine pools of either the symbiotic algae or the host (Table V). Only ± SD amol cell ' MSX was detected in algal pools after 24-h treatment of intact hydra, compared to high levels in freshly isolated symbionts after only 90 min of treat
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 214 P. J. McAULEY Treatment of freshly isolated symbionts at pH 5 caused a significant reduction in both glutamate and glutamine pools compared to those at pH 7 (one-way ANOVA, P <). Incubation of intact green hydra in MSX caused no significant change in the glutamate or glutamine pools of either the symbiotic algae or the host (Table V). Only ± SD amol cell ' MSX was detected in algal pools after 24-h treatment of intact hydra, compared to high levels in freshly isolated symbionts after only 90 min of treatment ( ± amol cell ' in freshly isolated symbionts treated at pH 5; ± amol cell ' in freshly isolated symbionts treated at pH 7). Treatment of intact hydra with AZS increased glutamine pools in both hydra and algae, although the increase was significant only in hydra. AZS treatment did not cause a reduction in either host or algal glutamate pools, in contrast to the consistent effect of AZS on glutamate pools measured in both freshly isolated symbionts and 3N813A irrespective of pH or nitrogen supply. Effect of MSX and AZS on glutamine ami methylamine uptake by 3N813A algae [uC]-glutamine uptake by 3N813A algae was competi- tively inhibited by a low concentration of AZS (Fig. 1). KM was ^M in the absence of AZS and /uA/ in the presence of AZS. In both cases, Vmax was 250 amol glutamine cell ' h '. There was linear uptake of the ammonium analog [I4C]- methylamine in the presence of both MSX and AZS, al- though uptake rates were reduced by and respectively, in comparison to controls (Fig. 2). In con- trast, ammonium chloride inhibited methylamine uptake Table V Effect ofinciihatmx hyilra in MSX or A7S on free s-lulamate and glutamine pooh t hvclra (pmol ng protein'1) and symbiotic algae (amol cell'1) - Control MSX AZS Hydra Glu ± ± ± Gin ± ± ± * Algae Glu ±
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
