. The Australian zoologist. Zoology; Zoology; Zoology. â¡ FREE â¡ ALBUMIN â GLOBULIN 0-10 I 10 I 40 TOTAL CONCENTRATION OF CORTISOL (jjg/IOOml 1âDistribution of Cortisol in the plasma of the echidna, brush-tailed possum and man. The absence of CBG in the echidna is evident from the low percentage of globulin-bound Cortisol and the failure to saturate the binding sites. Data from Khin Aye Than and McDonald (1976) and Tait and Burstein (1964) were used to calculate distribution in possum and human plasma respectively. Binding of 5a-dihydrotestosterone (DHT), testosterone (Te) and 17B-estradiol (
. The Australian zoologist. Zoology; Zoology; Zoology. â¡ FREE â¡ ALBUMIN â GLOBULIN 0-10 I 10 I 40 TOTAL CONCENTRATION OF CORTISOL (jjg/IOOml 1âDistribution of Cortisol in the plasma of the echidna, brush-tailed possum and man. The absence of CBG in the echidna is evident from the low percentage of globulin-bound Cortisol and the failure to saturate the binding sites. Data from Khin Aye Than and McDonald (1976) and Tait and Burstein (1964) were used to calculate distribution in possum and human plasma respectively. Binding of 5a-dihydrotestosterone (DHT), testosterone (Te) and 17B-estradiol (E2) DHT, Te and E2 were bound to a saturable globulin component of echidna plasma. The association constant was high for E2 and DHT but much lower for Te (Table 1). The concentration of binding sites was lowest for E2 and highest for Te. Binding to the albumin fraction, measured as the binding constant K> [£ a] (Tait and Burstein, 1964), was in the following order of decreasing strength: E2 > DHT > T. Increasing the temperature from 4° to 30°C decreased the association con- stants by 50-64% and the albumin binding constants by 32-38%. Specificity The ligand specificity of the binding protein(s) was examined by measuring the competitive displacement of a radioactive tracer of one of the three gonadal steroids by a second non-radioactive steroid. Table 2 shows that the bound: free ratio of Te, DHT and E2 decreased in the presence of a second non-radioactive steroid. However Te and E2 were displaced to a lesser extent than DHT, even at the high concentrations of steroids used for this experiment. 89. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Royal Zoological Society of New South Wales; Royal Zoological Society of New South Wales. Proceedings. [Sydney, Royal Zoological Society of Ne
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectzoology, bookyear1914
