. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. .Ch 1 mm 2. HmfL Fig. 107 (left). — Dematium. Bouin's method, stained with hemalum. 1, filament. 2, germinating conidium. VCM, vacuole containing metachromatin precipitates, n, nucleus. Fig. 108 (right). — Saprolegnia. Chondriome and vacuolar system. 1-3, Vital staining with neutral red. 1, tip of filament; reticular vacuole (RV) ; other elements omitted. 2, older filament; tendency of network to become a diffusely stained canal (V) containing deeply stained bodies (CM), other elements visible but unstained. 3, still older
. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. .Ch 1 mm 2. HmfL Fig. 107 (left). — Dematium. Bouin's method, stained with hemalum. 1, filament. 2, germinating conidium. VCM, vacuole containing metachromatin precipitates, n, nucleus. Fig. 108 (right). — Saprolegnia. Chondriome and vacuolar system. 1-3, Vital staining with neutral red. 1, tip of filament; reticular vacuole (RV) ; other elements omitted. 2, older filament; tendency of network to become a diffusely stained canal (V) containing deeply stained bodies (CM), other elements visible but unstained. 3, still older filament; vacuolar canal (V) loses its stain, other bodies as in (2). 4, mature filaments. Regaud's method with iron haematoxylin; vacuolar canal unstained, lipide granules dissolved, chondriosomes (Ch) strongly stained. 5, 6, mature filament. Meves' method with acid fuchsin; chondriosomes (Ch) red, lipide granules (Gg) brown. Ch, chondriosomes. N, nucleus. It must be added that the change to red in the vacuoles may also depend on the chemical constitution of colloidal substances which the vacuoles hold in solution. Mucilaginous substances, agar, for instance, change cresyl blue to a color toward the red, no mat- ter what the pH. According to LisoN, this color change in vital staining is a histochemical reaction characteristic of all sulfuric esters of high molecular weight. Now, mucilages or polyholoside esters are the most important among them. From these considerations it follows, therefore, that the change toward red of the vacuoles, which is due to the most variable causes, and which may be obtained in vitro by the addition of the most diverse substances, can not possibly serve to characterize a chemical substance. In general, the vacuolar colloids appear in the higher plants to be protein substances, perhaps proteins soluble in alcohol, and. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - co
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Keywords: ., bookcentury1900, bookcollection, booksubjectplantcellsandtissues
