. Seedling blight and stack-burn of rice and the hot-water seed treatment. in size, and a growth of Alternaria appeared on the also was isolated from rice seed grown in Louisiana. In Louisiana a leaf spotting of seedlings was noticed as beingquite common before the irrigation water was applied. The spotswere small, indefinite in outline, and light brown in color. An Alter-naria was found constantly associated with those spots. By examin -ing carefully under the microscope these young leaves, cleared bysoaking in equal parts of 95 per cent alcohol and glacial acetic acid, anA


. Seedling blight and stack-burn of rice and the hot-water seed treatment. in size, and a growth of Alternaria appeared on the also was isolated from rice seed grown in Louisiana. In Louisiana a leaf spotting of seedlings was noticed as beingquite common before the irrigation water was applied. The spotswere small, indefinite in outline, and light brown in color. An Alter-naria was found constantly associated with those spots. By examin -ing carefully under the microscope these young leaves, cleared bysoaking in equal parts of 95 per cent alcohol and glacial acetic acid, anAlternaria spore could be seen in the center of almost every spot, andoften the germ tube could be seen extending from the spore andpassing through the epidermis into the leaf tissues (Fig. 2). AnAlternaria leaf-spot of black Italian rice and of C. I. No. 15G4 wasfound at Belhiower station, near Los Angeles, Calif., but it was notdetermined whether or not this was identical with the form producingthe blast of rice kernels in the Sacramento Valley or with the3866°—22 2. 6 BULLETIN 1116, U. S. DEPARTMENT OF AGRICULTURE. Alternaria producing the seedling leaf-spot and seed injury inLouisiana. Rice infected with the various forms of fungi which produce fleck-ing and decay germinates very poorly as compared with uninfectedseed. The germ often is killed by the invading parasite (Pis. II andIII, A). Wet soils, which tend to check the normal germination anddevelopment of seedlings, seem to favor the growth of the fungus,and the seed may be destroyed and a poor stand result. Seed treat-ment before sowing would not eliminate injury done by these fungipreviously, but might prevent further injury and prevent the spreadof these organisms. Seed of Honduras rice obtained from California,where seed-infesting fungi are rare, and sown in Louisiana germinatedbetter than seed of the same variety grown in Louisiana (Tables 2and 4). The difference in the percentages of germination is anindication,


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