Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . ing the cocci. Bacterial Diagnosis.—By means of lumbar puncture thespinal fluid is obtained and allowed to settle. Smears madefrom sediment. Examined for bacteria. Gram-positive organisms are either pneumococci, strepto-cocci, or staphylococci. Meningococcus is Gram negative and within the leukocytes,and can be readily grown on blood-serum. If such an or-ganism is present, the disease is undoubtedly cerebrospinalmeningitis. Bacillus of Soft Chancre, Chancroid (Ducrey-Un
Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . ing the cocci. Bacterial Diagnosis.—By means of lumbar puncture thespinal fluid is obtained and allowed to settle. Smears madefrom sediment. Examined for bacteria. Gram-positive organisms are either pneumococci, strepto-cocci, or staphylococci. Meningococcus is Gram negative and within the leukocytes,and can be readily grown on blood-serum. If such an or-ganism is present, the disease is undoubtedly cerebrospinalmeningitis. Bacillus of Soft Chancre, Chancroid (Ducrey-Unna,1889).—^A diplobacillus which is specific has been describedby Ducrey as obtained from the secretion and in the depthand margins of the chancroid. Unnas bacillus is narrowerand unbroken in the center (Fig. 88). GONOCOCCUS.—MENINGOCOCCUS 179 Cultivation.—Cultivation has occurred on blood-agar, theblood being added in the proportion of one to two. Coloniesare small, round globules. Staining.—With borax, methylene-blue, decolorized withweak acetic acid. Pathogenesis.—Probably a mixed infection occurs in most. Fig. 88.—Smear of pus of chancroid of penis (X 1500) (Davis) (photo-micrograph by Mr. L. S. Brown). chancroids, especially if buboes result. The bacillus ofDucrey is not found in unopened buboes, though often con-taminating the ulcerated ones. The disease has been reproduced by inoculation of thehuman subject. Laboratory animals are immune. l80 ESSENTIALS OF BACTERIOLOGY CHAPTER XXV ANAEROBIC BACTERIA (BACILLUS OF TETANUS; BACILLUSOF MALIGNANT EDEMA, ETC.) Similar in form and cultural requirements are a group ofbacteria which are found as a result of injury or the infectionof wounds. They vary greatly in the clinical symptomsproduced. Bacillus of Tetanus (Nicolaier-Kitasato).—Origin.—Nicolaier found this bacillus in the pus of a wound in onewho had died of tetanus, describing it in 1884. Kitasato isolated and cultivated this germ (1889). Form.—A very s
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