. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. I I I I I I I I I I I I I I -7 -6 -5 -4 log [5HT (M)] B y O en I I .1 LJ_ I I I I I I I -8 -7 -6 -5 -4 -3 log [5HT (M)] Figure 4. (A) Dose-response curves for the inhibitory effect of 5-hy- droxytryptamine (SHT) on frontal cilia. Ordinate: the adjusted fractional iliftt'irnce: |l+ (fractional treatment rate - fractional control rate)]; ex- plained in the Methods section. The regression lines were derived from measurements made every 10 min after the addition of 5HT, continuing to M) min. Data
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. I I I I I I I I I I I I I I -7 -6 -5 -4 log [5HT (M)] B y O en I I .1 LJ_ I I I I I I I -8 -7 -6 -5 -4 -3 log [5HT (M)] Figure 4. (A) Dose-response curves for the inhibitory effect of 5-hy- droxytryptamine (SHT) on frontal cilia. Ordinate: the adjusted fractional iliftt'irnce: |l+ (fractional treatment rate - fractional control rate)]; ex- plained in the Methods section. The regression lines were derived from measurements made every 10 min after the addition of 5HT, continuing to M) min. Data points are omitted for clarity. (B) The regression line at 60 min with data points included. YFAFPRQamide was about 5 X 1(T7 M. At the maximum dose of YFAFPRQamide used (3 X 1(T6 M), there was about a 30% difference between the controls and the treat- ment cilia. This means that the cilia returned to their original rates because the 5HT did not completely inhibit them. The effects of the peptide did not appear until 45 min after the addition of 5HT, and there was no statistical difference between the regression lines at 45 and 60 min (F|3 _,-,, = 0. 1 , P = O.'M; Fig 5 A). The mean EC, 10 (± X Particle irunspiirt rules. Particle transport rates of both treated and control cilia varied from to mm/s (n = 1560 on 52 pieces of gill), but the rates were never zero in any of the experiments. Identification of SCPs in clam gill Radioimmunoassay. The purification of SCPs from clam extracts is strongly influenced by two features of the longest SCP, lAMSFYFPRMamide: First, even as the purification proceeds, this peptide is degrading by stepwise cleavage at log [YFAFPRQa (M)] B I °'2 2 05 J log [YFAFPRQa (M)] Figure 5. A) Dose-response curves illustrating the antagonistic effect of YFAFPRQamide on the 5-hydroxytryptamine- (5HT-) induced inhibi- tion of frontal cilia. Ordinate: the fracti
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
