. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. <&ZZZZgg2SZZ ^^^^^ tteiiii ^â ^^V:^^\':^.^:^â ^:^ '. ' ' T ! ' ' . ? ! V. -. ' ' ' RECOVERING INFECTIVE NEMATODE LARVAE FROM CULTURES G. F. White, U. S. Bureau of Entomology and Plant Quarantine THE method outlined here for recovering infective nematode larvae from cultures makes use of the often-observed fact that toward the close of their free-living period the larvae migrate from the medium in which they have been growing. The simple apparatus used t
. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. <&ZZZZgg2SZZ ^^^^^ tteiiii ^â ^^V:^^\':^.^:^â ^:^ '. ' ' T ! ' ' . ? ! V. -. ' ' ' RECOVERING INFECTIVE NEMATODE LARVAE FROM CULTURES G. F. White, U. S. Bureau of Entomology and Plant Quarantine THE method outlined here for recovering infective nematode larvae from cultures makes use of the often-observed fact that toward the close of their free-living period the larvae migrate from the medium in which they have been growing. The simple apparatus used traps the migrating larvae in water (White, 1927). Convenient and sufficient equipment consists of crystal- lizing dishes 125 to 150 mm. in diameter, watch glasses slightly larger than these di- mensions, petri dishes 100 to 125 mm., test tubes 20 by 150 mm., filter papers 9 to 12 cm., a spatula with a 4-inch blade, a test tube rack, a three-quart boiler with cover, animal charcoal, and sterile water. Brief steaming in the covered vessel suffices for the disinfection that is needed. The charcoal and feces with water added are mixed properly and con- veniently in one of the larger watch glasses and transferred to the half of a petri dish, with a moistened filter paper covering the bottom. Sterile water is poured into a crystallizing dish to cover the bottom and into it is placed the half petri dish containing the culture. A watch glass is used as a cover, the apparatus (Fig. 45) after labeling, is placed for incubation preferably where a high humidity may be maintained. Many of the larvae on approaching the third larval stage migrate from the culture and are trapped in the water surrounding the petri dish. In 166 Fig. 45.âApparatus used for culturing nema- tode larvae, a, Crystallizing dish; b, Petri dish with charcoal-feces mixture; c, watch-glass cover. Water surrounds the petri dish equal to about one half its Please note that these images are extracted from scanned
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Keywords: ., bookcentury1900, bookcollectionameri, bookcollectionbiodiversity
