. Biological structure and function; proceedings. Biochemistry; Cytology. 114 E. S. CANELLAKIS AND EDWARD HERBERT TABLE I Summary of Purification Procedure Specific mg. Protein activity* Yield RNA mg . RNA % of soluble cytoplasm mg Soluble cytoplasm 5-IO " 100" 135 100 Ammonium sulphate (o-55-0-86) 30-50 80 70 60 pH 5 precipitate 100-150 60 35 15 Hydroxylapatite Ribonucleoprotein a 80-100 4 3 I /3 750-1000 15-20 15-20 I y 400-600 10-15 10-15 I DEAE-cellulose "protein " 750 50 â 100 "RNA" â â 8-10 0-05 * The specific activity unit is expressed as /x//moles of [^*C]
. Biological structure and function; proceedings. Biochemistry; Cytology. 114 E. S. CANELLAKIS AND EDWARD HERBERT TABLE I Summary of Purification Procedure Specific mg. Protein activity* Yield RNA mg . RNA % of soluble cytoplasm mg Soluble cytoplasm 5-IO " 100" 135 100 Ammonium sulphate (o-55-0-86) 30-50 80 70 60 pH 5 precipitate 100-150 60 35 15 Hydroxylapatite Ribonucleoprotein a 80-100 4 3 I /3 750-1000 15-20 15-20 I y 400-600 10-15 10-15 I DEAE-cellulose "protein " 750 50 â 100 "RNA" â â 8-10 0-05 * The specific activity unit is expressed as /x//moles of [^*C]-AMP incorporated into S-RNA in the presence of i mg. protein in 6 min. at 25°.. Buffer cone Volu 280 ml. Fig. I. Stepwise procedure for elution of ribonucleoproteins from an hydroxy- apatite column. The width of the steps in the chromatogram represents the volume of eluate collected in each fraction. These volumes are as follows in the order of potassium phosphate buffer pH 7 â 2 concentrations listed under the chromatogram (0-05 M, o-o8 M, and 0-12 m); 20 ml., 10 ml., and 25 ml. Column dimensions 2 '5 cm. X 20 Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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