The Journal of biological chemistry . nisms chosen as representing themucoid group of bacteria were Friedlanders bacillus^ and Pfeiffersbacillus. They were grown on Prazmowskis medium in KoUeflasks with 1 per cent of the three sugars, dextrose, lactose, andsaccharose, added. This medium has the following composition: gm. Dipotassium phosphate Magnesium sulfate Ammonium carbonate Calcium chloride Water 1,000 cc. Profuse growth was always obtained after 48 hours incubationat 37°C. This was removed with a sterile right-angled glass rod,dried, desiccated, and-added to the ration in
The Journal of biological chemistry . nisms chosen as representing themucoid group of bacteria were Friedlanders bacillus^ and Pfeiffersbacillus. They were grown on Prazmowskis medium in KoUeflasks with 1 per cent of the three sugars, dextrose, lactose, andsaccharose, added. This medium has the following composition: gm. Dipotassium phosphate Magnesium sulfate Ammonium carbonate Calcium chloride Water 1,000 cc. Profuse growth was always obtained after 48 hours incubationat 37°C. This was removed with a sterile right-angled glass rod,dried, desiccated, and-added to the ration in place of 5 per cent ofstarch. The diets and results of the feeding tests are shown inFigs. 2 and 3. It will be noted from Fig. 2 that the animals con-tinued to lose during the second period; , while Friedlandersbacillus was added to the ration. In the case of Pfeiffers 1 The culture here referred to is a mucoid organism carried in this labora-tory under the name Friedlanders bacillus, but fermenting lactose. Samuel R. Damon 899. Tdaus Diet Period 1 ?Perio Casein 18 18 SuqaP n 17 Slapch 5Z 47 Salt riixturel83 3 3 Bv/11eT>(at 10 10 B . JTiecHaT der 5 Fig. 2. During the second period 5 per cent of Friedlanders bacillusreplaced an equivalent amount of starch. The curves would not seem toindicate the presence of any vitamin in the bacteria.
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectbiochem, bookyear1905
