. The microscope; an introduction to microscopic methods and to histology. Microscopes. â Exc Fig. 135. View of the Back Lens OE THE Objective, Showing the Con- denser OUT of Center and Centered. Exc The spot of light {D) is to one side of the center, showing that the optic axis of the condenser is not in line with that of the microscope. C The spot of light {D) is in the center, showing that the optic axis of the con- denser and microscope are in line. A plan used by many skilled workers is to light the object and the field around it well and then to place a metal diaphragm of the proper size
. The microscope; an introduction to microscopic methods and to histology. Microscopes. â Exc Fig. 135. View of the Back Lens OE THE Objective, Showing the Con- denser OUT of Center and Centered. Exc The spot of light {D) is to one side of the center, showing that the optic axis of the condenser is not in line with that of the microscope. C The spot of light {D) is in the center, showing that the optic axis of the con- denser and microscope are in line. A plan used by many skilled workers is to light the object and the field around it well and then to place a metal diaphragm of the proper size in the camera very close to the plate holder. This will insure a clean, sharp margin to the picture. This metal dia- phragm must be removed while focusing the diaphragm of the projection ocular, as the dia- phragm opening is smaller than the image of the ocular dia- phragm. If the young photo-microg- rapher will be careful to select for his first trials objects of which really good photo-mi- crographs have already been made, and then persists with each one until fairly good results are attained, his progress will be far more rapid than as if poor pictures of many different things were made. He should, of course, begin with low magnifications. § 353. Adjusting the objec- tive for cover-glass. â After the object is properly lighted, the objective, if adjustable, must be corrected for the thickness of cover. If one knows the exact thickness of the cover and the objective is marked for different thick- nesses, it is easy to get the adjustment approximately cor- rect mechanically; then the final corrections depend on the skill and judgment of the worker. It is to be noted too that if the objective is to be used without a. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Gage, Simon Henry, 1851-1944. [Ithaca,
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