. Bulletin. Natural history; Natural history. 456 Natural History Survey Bulletin Vol. 26, Art. 6 havior traceable to size or character of in- oculum : coarse white cotton thread was cut in pieces about H-j inches lonjr and autoclaved in distilled water. These pieces were laid on an agar slant, which was then inoculated from the isolate to be tested. About a week after fungus growth had overrun the cotton threads, the threads were lifted from the culture, scraped clean of adhering agar, and cut with sterile scis- sors into sections, each about 2 milli- meters long. One piece of Fusa
. Bulletin. Natural history; Natural history. 456 Natural History Survey Bulletin Vol. 26, Art. 6 havior traceable to size or character of in- oculum : coarse white cotton thread was cut in pieces about H-j inches lonjr and autoclaved in distilled water. These pieces were laid on an agar slant, which was then inoculated from the isolate to be tested. About a week after fungus growth had overrun the cotton threads, the threads were lifted from the culture, scraped clean of adhering agar, and cut with sterile scis- sors into sections, each about 2 milli- meters long. One piece of Fusarium-'in- fested thread was placed in the center of each dish. Immediate'.v after inoculation the dishes were placed in electrically con- trolled incubators kept at temperatures shown in table 2 and left for 160 hours. At the end of the incubation period the of the colonies were measured, table 2. Two dishes of each isolate were used for each temperature. Relative sizes of the colonies grown at different temper- atures are shown in figs. 5, 6, 7, and 8. None of the isolates grew at the 1-3 de- gree C. temperature range, table 2, and only brown rot isolate 45-75 and vascular isolate 50-24 showed traces of growth at 5 degrees C Since the next higher tempera- nmm m€(y. Fig. 5.—Six isolates of Fusarium grown on Coons's agar for 160 hours at the following tem- peratures: 20-22, 24, 27-28 degrees C. (top row in each set of six dishes, left to right) ; 30, 32, and 36 degrees C. (bottom row in each set, left to right). Isolates 45-73 and 50-24 are vascular isolates, 45-75 and 50-22 are brown rot isolates, 47-3 and 50-23 are basal dry rot Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Illinois. Natural History Survey Division. Urbana, State of Illinois, Dept. of Registration and Education,
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