. Chart. Stations in the Gulf of Aden occupied by 'Mabahiss', 'Discovery II' and Mr Fraser-Brunner. solution of magnesium chloride makes it possible to achieve a good deal of relaxation of muscles and consequent expansion before killing in formalin, which should contain at least 5 % formaldehyde. It is advisable to add 2 lb. of hexamine (hexamethylenetetramine) to each gallon of 38-40 % formalde- hyde solution to buffer it. Before subsequent examination it is a good plan to stain for a day or two with dilute Grenacher's Borax-carmine. A little of this stain can be added to the jar in which the
. Chart. Stations in the Gulf of Aden occupied by 'Mabahiss', 'Discovery II' and Mr Fraser-Brunner. solution of magnesium chloride makes it possible to achieve a good deal of relaxation of muscles and consequent expansion before killing in formalin, which should contain at least 5 % formaldehyde. It is advisable to add 2 lb. of hexamine (hexamethylenetetramine) to each gallon of 38-40 % formalde- hyde solution to buffer it. Before subsequent examination it is a good plan to stain for a day or two with dilute Grenacher's Borax-carmine. A little of this stain can be added to the jar in which the specimens are preserved. For examination of the external form of nectophores and bracts, brief staining in dilute Delafield's haematoxylin is effective, but if hexamine has been used the specimens must first be thoroughly washed or the stain will be precipitated. To photograph them alive is exceedingly difficult because of the lack of contrast, the depth of the field that it is necessary to cover, and their constant change of form and position. The text-figures, with the exception of a few diagrammatic ones, were all drawn by the author with the aid of a camera lucida. The inking in of the figures was done by Mrs Walker (ne'e E. C. Humphreys) after demonstration and examination of the specimens. A subsequent check with the specimens was made by myself, or by both of us. In order to be able to make drawings of large specimens I removed the foot of a Leitz binocular and made the stage slide on a track, fixed at such a height above the
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Keywords: ., bookcentury1900, booksubjectocean, booksubjectscientificexpediti
