. Biological structure and function; proceedings. Biochemistry; Cytology. 132 KEITH R. PORTER chromophilic material of the liver cell cytoplasm (1943). A definitive demonstration of the intracellular origin of the microsome v^'as not, how- ever, provided until about 12 years later when Palade and Siekevitz [14] published micrographs of sections through the pellet representing the. Fig. 2. Micrograph of thin section of microsome pellet from rat liver homo- genization and fractionation. The long slender profiles are readily identified as fragments of intracellular, particle-studded (rough) ciste
. Biological structure and function; proceedings. Biochemistry; Cytology. 132 KEITH R. PORTER chromophilic material of the liver cell cytoplasm (1943). A definitive demonstration of the intracellular origin of the microsome v^'as not, how- ever, provided until about 12 years later when Palade and Siekevitz [14] published micrographs of sections through the pellet representing the. Fig. 2. Micrograph of thin section of microsome pellet from rat liver homo- genization and fractionation. The long slender profiles are readily identified as fragments of intracellular, particle-studded (rough) cisternae of the endoplasmic reticulum (see Fig. 4 for comparison of rough ER in intact cell). There are also in the field (at arrows) a few profiles of vesicles without particles (smooth or agranular). These are thought to be derived from the smooth form of the reticulum observed in liver cells. Mag. x 70 000. (Micrograph courtesy of G. E. Palade.) microsome fraction. These pictures showed profiles ot particle-studded units which by their morphology could be unmistakably identified as fragments or pieces of the endoplasmic reticulum of the intact rat liver cell (Fig. 2). Thus the microsome became part of a well characterized. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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