. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. BUFFERING POWERS OF HELIX POMATIA SERUM S27 ( Van Slyke et al., 1922). For unit change in pll, ft therefore is the amount of acid or base required to effect this change. The titration curves above give the buffering power of the hemocyanin containing solutions in terms of mgm. Cu per cc. and 10 3 millimols acid or base. The slopes of these curves have been determined at intervals of pH, and the results plotted in Fig. 4. The figure shows the higher buffering power of the serum containing mgm. Cu per cc., but t
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. BUFFERING POWERS OF HELIX POMATIA SERUM S27 ( Van Slyke et al., 1922). For unit change in pll, ft therefore is the amount of acid or base required to effect this change. The titration curves above give the buffering power of the hemocyanin containing solutions in terms of mgm. Cu per cc. and 10 3 millimols acid or base. The slopes of these curves have been determined at intervals of pH, and the results plotted in Fig. 4. The figure shows the higher buffering power of the serum containing mgm. Cu per cc., but the correspondence of the shapes of the curves and the points of minimal and maximal buffering show that for all three samples of fresh serum the same buffering systems are involved. X "o FIG. 5. Buffering power of Roche hemocyanin (represented by solid circle) ; of dialysed oxygenated serum May 2 (represented by cross) ; and of dialysed oxygenated serum July 23 (represented by triangle). DISCUSSION The chloroform-treated, dialysed solutions used in the experiments were not pure isoelectric oxyhemocyanin in solution, since the pH's of the two preparations were and , and Hcli.\- poniatia hemocyanin has been shown to have an isoelectric point of (Tiselius, quoted from Roche, 1931, and Stedman and Stedman, 1927). Proteins in the blood other than hemocyanin might be expected to exert a buffer effect, and would not be removed by dialysis. Cuenot (1892) found that in blood there were no proteins precipitated by acetic, as in the case of crustacean and cephalopod blood, and further that all the protein. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
