. The microscope; a simple handbook. Microscopes. APPARATUS FOR HOLDING SPECIMENS 59 the red or white, or with a solution of acetic acid when a count of white cells only is being made. In counting red corpuscles a dilution of 1-200 is generally used, but in certain cases 1-100 may be employed. The blood is drawn into the pipette up to the mark 0*5 in the case of 1-200 dilutions, and up to the mark 1 for 1-100 dilutions. The pipette is then immediately placed in the. Fia. 60.âNo. 3325a, Pipettes for Red and White Corpusdes. diluting fluid, which is drawn up to the mark 101 above the bulb. Both
. The microscope; a simple handbook. Microscopes. APPARATUS FOR HOLDING SPECIMENS 59 the red or white, or with a solution of acetic acid when a count of white cells only is being made. In counting red corpuscles a dilution of 1-200 is generally used, but in certain cases 1-100 may be employed. The blood is drawn into the pipette up to the mark 0*5 in the case of 1-200 dilutions, and up to the mark 1 for 1-100 dilutions. The pipette is then immediately placed in the. Fia. 60.âNo. 3325a, Pipettes for Red and White Corpusdes. diluting fluid, which is drawn up to the mark 101 above the bulb. Both ends of the pipette are then closed with the fingers, and the pipette shaken to ensure an even mixing, the glass bead in the bulb facilitating this. For white corpuscles, a dilution of 1-10 is employed and the other pipette is used. For filling the counting chamber, a drop of the mixture is blown out of the pipette, after allowing several drops to go waste, into the centre of the counting chamber. The cover glass is then placed over the cell. The drop of blood must not be allowed to overflow the platform into the groove which surrounds it, and the cover glass must be in perfect contact with the object slide, and aU must be scrupulously clean. B I -Uj- C _i_ 1 Fig. 61. Depth â 1 400 cAb THOMA HAWKSLEY >* Fig. 62.âNo. 3325a, Thoma-Hawksley Counting Chamber. The counting chamber consists of a plate of glass with an aimular groove ground upon it. The circular portion inside the groove is ground and polished to a distance of '1 mm. below the level of the plate of glass. In the Thoma hsemacytometer this portion is ruled with a diamond into squares l/400th of a square mm. each in area. It. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Beck, Conrad, 1864-. London, R. & J. Beck, Ltd.
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Keywords: ., bookcentury1900, bookdecade1920, booksubjectmicroscopes, bookyear
