. The Biochemical journal, 1918. Biochemistry. 504 W. J. YOUNG The figures are shown graphically on the accompanying M 0-Z 0-3 04 0-5 M 0-7 0-8 0-9 1-0 5ERUM ADDID CC 2-0 They show further that the sheep serum produced a much greater inhibi- tory effect than did the dog serum, both in the early stages and in the final rate. It is apparent, however, that a quantitative comparison of the actions of the two sera cannot be obtained by adding a definite amount of serum to a trypsin digest and comparing the action, since the comparison depends upon which part of the curves representing the
. The Biochemical journal, 1918. Biochemistry. 504 W. J. YOUNG The figures are shown graphically on the accompanying M 0-Z 0-3 04 0-5 M 0-7 0-8 0-9 1-0 5ERUM ADDID CC 2-0 They show further that the sheep serum produced a much greater inhibi- tory effect than did the dog serum, both in the early stages and in the final rate. It is apparent, however, that a quantitative comparison of the actions of the two sera cannot be obtained by adding a definite amount of serum to a trypsin digest and comparing the action, since the comparison depends upon which part of the curves representing the reaction are used. A similar experiment was done with another sample of trypsin to try the effect of very small quantities of serum. The sheep serum was diluted ten times with water, and the dog serum with an equal amount of water. In each case 2 cc. trypsin were employed and 50 cc. caseinogen, the total volume being 55 cc. The mixtures were incubated for four hours at 37°. The figures in the first column give the volumes of undiluted serum contained in the mixtures. Soluble nitrogen produced Serum added ^ cc. Sheep serum Dog serum 0 11-8 11-8 0-02 12-2 — 0-04 11-6 12-5 0-05 — 12-5 006 10-3 — 0-08 9-5 — 010 8-6 12-3 0-15 6-5 — 0-20 4-9 11-8 0-25 3-6 — 0-4 — 9-2 10 — 40 1 cc. trypsin in absence of serum gave 5-5 Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Biochemical Society (Great Britain); University of Liverpool. Biochemical Dept. London [etc. ] Cambridge University Press
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