. Comparative animal physiology. Physiology, Comparative; Physiology, Comparative. 670 Comparative Animal Physiology strong support to the view that the aetual Hght-emitting molecule is the luciferin. All our present knowledge of the properties of the bioluminescent process appear capable of fitting into the following scheme of reactions.^"'- ^" Let A stand for luciferase, LH- for reduced luciferin (max. absorption, 435 m/x) and L for its oxidation products (max. absorption 465 m/x), L'^ for excited luciferin, L, for destroyed luciferin. and X for an unknown fh. donor, possibly gluco
. Comparative animal physiology. Physiology, Comparative; Physiology, Comparative. 670 Comparative Animal Physiology strong support to the view that the aetual Hght-emitting molecule is the luciferin. All our present knowledge of the properties of the bioluminescent process appear capable of fitting into the following scheme of reactions.^"'- ^" Let A stand for luciferase, LH- for reduced luciferin (max. absorption, 435 m/x) and L for its oxidation products (max. absorption 465 m/x), L'^ for excited luciferin, L, for destroyed luciferin. and X for an unknown fh. donor, possibly glucose. 1. 2. 3. 4. 5a. 5b. 5c. 6. 7. AL + A + LH.— :ALH. + X ALH. + O.^ ALH . =^ALU + H(X. AL + LH AL + LH AL- + LH ALH + O. AL + CX — :AL + H^ >AL* + LH- >AL + LH *AL, +LH- -* AL + LH- + hv -* AL + HO. -*AL, Essentially the same reactions, with the exception of reaction 2, also appear to proceed in the absence of A, but their rates, especially that of reaction 5, are such that no light is then produced. It is readily apparent from the foregoing scheme of reactions that only reaction 5a is the actual lioht-emittinw one, but that this reaction is de pendent on, or associated with, a number of other reactions which b\ them- selves are dark reactions. The whole problem at the moment appears now to be in such a state that one cannot sav with llnalitv whether it is lucifer-. "* '^ §\ \ / A / ^ V c \ \ k^— ^ \ Mo MbO SOO 5M0 no '»60 SOO 540 WAVELENGTH- mid I iK- 25.^. C'lianKCs in the ahsoriition S|xctriiiii ol liiciliTin: A, iliiiinj; oxiilatinn with DXNKfn uitliout hicilcrase (non-luniincscent), and H, in the presence ol lucilerase (hj^ht |>r(itkicinK)- '" A eiirves H throuKli N were obtained after 9, 20, .-^0, 39. 49, 455, and 1 140 minutes, respectively. In />, curves \\ throus^li \ were obtained at 22, 40, and 457 minutes. From (ohnson.''. Please note that these images are extracted from scanned page images that may have been digitall
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