. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. CHL-770 FROM GREEN BACTERIA 427 SEDIMENTATION COEFFICIENT AND MOLECULAR WEIGHT The sedimentation coefficients at four different protein concentra- tions were measured from the sedimentation velocity in the Spinco analytical ultracentrifuge, Model E, The chlorophyll-protein was dialyzed against iV NaCl in M Tris, pH , and sedimented at 59,780 rpm in a double-sector cell. The Schlieren pattern (see Fig, 4) was Dhotographed at 8-minute intervals, and the distance of the peak. Fig. 4. Schlieren pattern of the descendin
. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. CHL-770 FROM GREEN BACTERIA 427 SEDIMENTATION COEFFICIENT AND MOLECULAR WEIGHT The sedimentation coefficients at four different protein concentra- tions were measured from the sedimentation velocity in the Spinco analytical ultracentrifuge, Model E, The chlorophyll-protein was dialyzed against iV NaCl in M Tris, pH , and sedimented at 59,780 rpm in a double-sector cell. The Schlieren pattern (see Fig, 4) was Dhotographed at 8-minute intervals, and the distance of the peak. Fig. 4. Schlieren pattern of the descending boundary in the double-sector cell after approximately 30 minutes of centrifugation at 59,780 rpm. A cm-1, t - °C. 809 from the center of rotation was plotted versus time on a semilog scale. The sedimentation coefficient was calculated from the slope of the best straight line fitted to the data according to the usual equation and then corrected for solvent viscosity and temperature (9). The corrected values for S2o w ''â ^ '^ concentrations of material are shown in Fig. 5, The linear extrapolation to zero concentration was fitted to the experimental points by the method of least squares. In spite of the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Symposium on Bacterial Photosynthesis (1963 : Yellow Springs, Ohio); Charles F. Kettering Research Laboratory (Yellow Springs, Ohio); Gest, Howard. Yellow Springs, Ohio, Antioch Press
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