. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 3B Figure 3. Glycoproleins of the Ctirciniix nuiciuix cephalolhoracic shield cuticle separated on SDS-poly- acrylamide gels (15r/r. 25 /Mg protein/lane), electroblotted to PVDF, and probed with Con A (lanes M and I to 4) in the presence of MMP (lanes 5 and 6) and with previous deglycosylation by /V-glycosidase F (lanes 7 and 8). Fig. 3A. Blotting membranes Lane M: pre-stamed Molecular Weight Marker Molecular Weight Range (GibcoBRLl with labeled 31- and 45-kDa bands; Lane I: 2c/c HAc-e\tracl: Lanes 2. 5.
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 3B Figure 3. Glycoproleins of the Ctirciniix nuiciuix cephalolhoracic shield cuticle separated on SDS-poly- acrylamide gels (15r/r. 25 /Mg protein/lane), electroblotted to PVDF, and probed with Con A (lanes M and I to 4) in the presence of MMP (lanes 5 and 6) and with previous deglycosylation by /V-glycosidase F (lanes 7 and 8). Fig. 3A. Blotting membranes Lane M: pre-stamed Molecular Weight Marker Molecular Weight Range (GibcoBRLl with labeled 31- and 45-kDa bands; Lane I: 2c/c HAc-e\tracl: Lanes 2. 5. and 7: N HCI-extract; Lanes 3. 6. and S: 10', EDTA-extract; Lane 4: ( M KCl-extract. Fig. 3B. Densitometric profiles of the HCI- and EDTA-e\tracts lanes (2 and 3). ones previously found to react with Con A (, the bands at 29, 30-32, 34. 40. 45. 50. 55. and 100 kDa in both extracts: at 10. 15 and 22-24 kDa in the HCI extract; and at 80-85 kDa in the EDTA-extract). but several Con A-pos- itive bands tailed to react with Jac (;.. the bands at 12. 15. 20. 32. and 200 kDa in the HCl-extract lane: the bands at 20. 24-26. 70. and 200-kDa in the EDTA-extract lane). Sambucus nigra agglutinin and Maackia amurensis ag- M HCI EDTA kDa -66 |-45 -31 -14. 4A Figure 4. (jlycoprolems i>l ihe Ciin-iini.\ imiciiti* cephalmhoiacic shield cuticle separated on ID SDS- polyacrylaniide gel (15'.}. 25 /ug protein/lane), electrohlottetl to PVDF. and probed with Jae. Fig. 4A. Lane M: pie-stamed Molecular Weight Marker Molecular Weight Range (GibcoBRLl with labeled and 45-kDa hands; Lane 1: N HCl-e\tract; Lane 2; Kl'r FDTA-extract. Fig. 4B. Densitometric Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
