. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 73 T 48 h 24 h Cs+ CI K+ Photoablation + CI Figure 4. Percent metamorphosis in larvae of PhcMillu \ih,>^iw that were stained with DASPEI, embedded in low-melting-point agarose, ex- posed for 20 mm to fluorescent irradiation aimed either at the apical sensory organ (ASO) or the foot, freed from the agarose. and then placed in coral inducer for 24 h (n = 3 replicates. 15 larvae/replicate!: bars = mean and SD. ASO, fluorescent light locused on the ASO; Foot, fluores- cent light focused on the foot. Not embedded in agarose we
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 73 T 48 h 24 h Cs+ CI K+ Photoablation + CI Figure 4. Percent metamorphosis in larvae of PhcMillu \ih,>^iw that were stained with DASPEI, embedded in low-melting-point agarose, ex- posed for 20 mm to fluorescent irradiation aimed either at the apical sensory organ (ASO) or the foot, freed from the agarose. and then placed in coral inducer for 24 h (n = 3 replicates. 15 larvae/replicate!: bars = mean and SD. ASO, fluorescent light locused on the ASO; Foot, fluores- cent light focused on the foot. Not embedded in agarose were larvae used in C, untreated controls; CI, untreated larvae exposed to coral inducer (H = 3 replicates, 25 larvae/replicate). lished personal observations on larvae of H\dnudes el- egans). larvae of a chiton (Barnes and Conor. 1973). and veligers of the abalone Haliolis rufescens (Wodicka and Morse, 1991) apparently detect absorbed settlement cues by brushing the surfaces of substrata with their apical ciliary. Figure 5. (A) Veliger larva of Phestilla sihagae that was stained with DASPEI. embedded in low-melting-point agarose. and subjected to exci- tatory irradiation focused on the apical sensor) organ (ASO) for 2(1 nun. The ASO cells are bleached (compare to Fig. 2B. C). although the DASPEI stain remains in other parts of the larval body. (B) The same larva after 20-min immersion in acridine orange in filtered seawater. The ASO cells emit a pale green fluorescence. Other bright areas, some not visible in both photos, retain DASPEI stain. Scale bars = 100 Photoablation (20 min) Figure 6. Percent metamorphosis in larvae of Phestilla sihogac that had undergone photoablation of apical-sensory-organ (ASO) cells and then exposure to the ionic inducers potassium or cesium; bars = mean and SD. Percent metamorphosis was determined after 24 h in all cases, plus at 48 h for potassium-treated larvae. Experimental treatments (larvae embedded in low-melting-point agarose for light
Size: 2194px × 1139px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
