. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 382 LEONARD NELSON nates: t]sp/C) at different concentrations of the sample dissolved in 10 ATP- ascorbate solution provide the data for the graph (Fig. 2). Protein concentrations, in ml., appear on the abscissa. Extrapolation of the curve to infinite dilution of the protein gives an intrinsic viscosity of about Samples dissolved in M KC1 prior to the step at which spermosin was removed by denaturation in cold alcohol were tested for sensitivity to ATP. This material, referred to as "flactospermosin,
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 382 LEONARD NELSON nates: t]sp/C) at different concentrations of the sample dissolved in 10 ATP- ascorbate solution provide the data for the graph (Fig. 2). Protein concentrations, in ml., appear on the abscissa. Extrapolation of the curve to infinite dilution of the protein gives an intrinsic viscosity of about Samples dissolved in M KC1 prior to the step at which spermosin was removed by denaturation in cold alcohol were tested for sensitivity to ATP. This material, referred to as "flactospermosin," had ATP-ase activity and underwent precipitation after 10-fold dilution with mM MgCl-j in the absence of ATP and superprecipitation on dilution in the presence of mM ATP. Two aliquots of flactospermosin in M KC1 had outflow times in the Ostwalcl viscometers of between three and four minutes. Addition of 1/10 volume of 2 mM ATP-ascorbate solutions caused rapid drops in the outflow time followed by slower STARFISH FLAGELLAR "FLACTO-SPERMOSIN" 190 P 180 S 170 160 - !r 150 - 140 - 130 - 120. 120 (MIN) FIGURE 3. Starfish flagellar flactospermosin. Effect of ATP on viscosity of M KG solutions of flactospermosin. Two-mi, samples of flactospermosin ; \\, volume of 2 mM ATP added; I\T volume of 4 mM MgCU added at arrow. Ordinate: outflow time in Ostwald viscometer. Abscissa: duration of experiment in minutes to indicate relative time required for "contraction" and "relaxation" showing ATP-sensitivity of flactospermosin. In terms of specific viscosity, deviations from the initial value of subsequent to additions of the various reagents and recoveries resulted in the following minima and maxima: , , , , and finally Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perf
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
