. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LARVAL RESPONSES TO SETTLEMENT CUES AND MICROBIAL FILMS 275 than the rate observed during a normal swim were con- sidered to be showing an increased ciliary beating. Larvae were directly placed on the films and observed with a Wild dissecting microscope; the number showing an ac- tivity response was recorded at intervals of 0-2, 2-4, 4- 6, 6-8, and 8-10 min (hereafter 2, 4. 6. 8. and 10 min, respectively). A total of 30 individual bioassays were car- ried out per film. A G test was used for statistical analysis. Results Larv
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LARVAL RESPONSES TO SETTLEMENT CUES AND MICROBIAL FILMS 275 than the rate observed during a normal swim were con- sidered to be showing an increased ciliary beating. Larvae were directly placed on the films and observed with a Wild dissecting microscope; the number showing an ac- tivity response was recorded at intervals of 0-2, 2-4, 4- 6, 6-8, and 8-10 min (hereafter 2, 4. 6. 8. and 10 min, respectively). A total of 30 individual bioassays were car- ried out per film. A G test was used for statistical analysis. Results Larval response to different natural and artificial cues Mucus and hemolymph. To learn about the effect of conspecific natural substances on the behavioral response of C. concholepas. early pre-competent larvae of this gas- tropod were exposed to mucus and hemolymph. In the presence of conspecific mucus, the veliger larvae stopped swimming and sank. The effect of mucus was time-de- pendent and reached a plateau after 5 min of continuous exposure to the film (Fig. 1). At this time, about 50% of the larvae were inactive on the bottom of the wells and remained in this state for 30 min of incubation (Fig. 1). Thereafter, the number of larvae swimming normally in- creased, leaving only 10% of the total larvae sunk after 2 h (Fig. 1). Larvae exposed to hemolymph showed a pro- nounced and quick response. After just 2 min of exposure, the sinking rate was 94% (Fig. 2). Thereafter, the number of larvae showing a cessation of swimming slowly de- creased, resulting in a 75% sinking rate after 30 min (Fig. 2). Only a few larvae remained inactive on the bottom (16%) after 2 h of incubation (data not shown). Larvae exposed to boiled hemolymph showed a response similar to that observed with normal hemolymph during the first 100. 120. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illus
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
