. The microscope : an introduction to microscopic methods and to histology. Microscopes. 2 go SECTIONING [(//. X The tissue is held by the microtome and moved up by means of a screw. The knife rests on the top of the microtome and is moved across the tissue by the hand. Microtomes of this kind are excel- lent. No one need wait for expensive automatic microtomes to do good sectioning. With a good table microtome the knife being guided by the hand or hands of the operator, he can make straight cuts as for paraffin sectioning, or drawing cuts as for collodion work. (Figs. 228-229).. Fig. 228 Fig.
. The microscope : an introduction to microscopic methods and to histology. Microscopes. 2 go SECTIONING [(//. X The tissue is held by the microtome and moved up by means of a screw. The knife rests on the top of the microtome and is moved across the tissue by the hand. Microtomes of this kind are excel- lent. No one need wait for expensive automatic microtomes to do good sectioning. With a good table microtome the knife being guided by the hand or hands of the operator, he can make straight cuts as for paraffin sectioning, or drawing cuts as for collodion work. (Figs. 228-229).. Fig. 228 Fig. 229 Figs. 228-229. Hand and table microtomes. Both have a screw Jot ele- vating the object to be cat and a surface on which to rest the section knife. 228 in held in the hand, 229 is fastened to a table. The knife is held and moved by the hand in both cases. ( Cuts loaned by the Bauch & Lonib Optical Co). § 438. Sectioning with a Freezing Microtome.—In this method of sectioning the tissue is rendered firm by freezing and the sections are cut rapidly by a planing motion as with paraffin. Now the most usual freezing microtome is one in which the freezing is done with escaping liquid carbon dioxid. The microtome is in general like the one shown in Fig. 229. The knife should be very rigid. A plane blade is often made use of. The tissue may be either fresh or fixed. If alcohol has been used it must be soaked out of the tissue by placing it in water. Sometimes tissues are. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Gage, Simon Henry, 1851-1944. Ithaca, N. Y. : Comstock Publishing Company
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