. Asiatic herpetological research. Reptiles -- Asia Periodicals; Amphibians -- Asia Periodicals. Vol. 5 p. 144 Asiatic Herpetological Research December 1993 {0<f27b5"432Li. FIG. 1. The Acidic PAGE of the different fractions after separation with a Sephadex G-50 column ( x 200 cm). 1: Crude venom; 2-11: Different fraction after separation with a Sephadex G- 50 column. Fractions 7-11 were collected together for the further isolation. containing NaCl gradient () and finally was eluted by the buffer containing NaCI gradient (). The two neurotoxins were eluted during the first
. Asiatic herpetological research. Reptiles -- Asia Periodicals; Amphibians -- Asia Periodicals. Vol. 5 p. 144 Asiatic Herpetological Research December 1993 {0<f27b5"432Li. FIG. 1. The Acidic PAGE of the different fractions after separation with a Sephadex G-50 column ( x 200 cm). 1: Crude venom; 2-11: Different fraction after separation with a Sephadex G- 50 column. Fractions 7-11 were collected together for the further isolation. containing NaCl gradient () and finally was eluted by the buffer containing NaCI gradient (). The two neurotoxins were eluted during the first gradient process. The neurotoxins from CM- Sephadex C- 25 still showed two other minor bands on acidic PAGE. So each of them was further purified on the CM- Cellulose 52 Column. The equilibrium buffer is , pH HAc-NaAc buffer. The NaCI gradient is in the same buffer. 2. Determination of purities: Acidic PAGE was used. The separating gel was 15 %, and the spacal gel was %. The gel was stained by R- 250 dissolved in 375 ml ethanol, 125 ml water and 5 ml methanol. 3. Determination of amino acid composition: The samples were analyzed with a Hitachi Model 835-50 High Speed Amino Acid Analyzer. 4. Determination of amino acid sequence of the neurotoxin: The method was in accordance with the method of Lin and Wang (1984).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Asiatic Herpetological Research Society; Chung-kuo liang chi pa hsing tung wu hs©eh hui. Berkeley, Calif. : Asiatic Herpetological Research Society : Chinese Society for the Study of Amphibians and Reptiles
Size: 1670px × 1496px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookcollectionbiodiversity, bookcontributorharv
