. Some diseases of Puerto Rican forage crops. Forage plants Diseases and pests Puerto Rico. Figure 25.—A stem spot on Stizolohium deeringianum (velvetbean), the cause of which has not been determined. X mm. Coalesced spots are much larger. On several occasions attempts were made to isolate the causal organism. Portions of young dis- eased tissue were isolated asepticall}^ and cultured at room temperature (74°-80° F.) on agar media (su- crose, 5 gm.; Difco veast extract, 3 gm.; K0HPO4, gm.; NaCl, gm.; ilgS04, gm.; CaCls, gm.; agar, 15 gm.; water, 1,000 cc; pH ). Most of

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. Some diseases of Puerto Rican forage crops. Forage plants Diseases and pests Puerto Rico. Figure 25.—A stem spot on Stizolohium deeringianum (velvetbean), the cause of which has not been determined. X mm. Coalesced spots are much larger. On several occasions attempts were made to isolate the causal organism. Portions of young dis- eased tissue were isolated asepticall}^ and cultured at room temperature (74°-80° F.) on agar media (su- crose, 5 gm.; Difco veast extract, 3 gm.; K0HPO4, gm.; NaCl, gm.; ilgS04, gm.; CaCls, gm.; agar, 15 gm.; water, 1,000 cc; pH ). Most of the pieces cultured had no growth of any kind. The fungi that grew were of vari- able types and were considered as secondary organisms. In addition, infected plants were kept in a moist chamber for several days. Vari- ous fungi were observed in older. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Theis, Thomas. Federal Experiment Station of the United States Dept. of Agriculture

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