Practical pathology; a manual for students and practitioners . us, may on careful examination be foundto be slightly granular. Near the posterior, or blunt, end is a secondbut much smaller deeply stained reddish-purple point known as themicro-nucleus or centrosonie. These organisms are from 15 to 25/xin length (without the continuing flagellum, which is from 5 to 6/x)and from 1*5 to 2-5 yu. broad. Around the micro-nucleus the protoplasmis not so deeply stained. Arising from or near this micro-nucleus and 770 MICROSCOPIC PARASITES running along the margin of the organism is a narrow band, which
Practical pathology; a manual for students and practitioners . us, may on careful examination be foundto be slightly granular. Near the posterior, or blunt, end is a secondbut much smaller deeply stained reddish-purple point known as themicro-nucleus or centrosonie. These organisms are from 15 to 25/xin length (without the continuing flagellum, which is from 5 to 6/x)and from 1*5 to 2-5 yu. broad. Around the micro-nucleus the protoplasmis not so deeply stained. Arising from or near this micro-nucleus and 770 MICROSCOPIC PARASITES running along the margin of the organism is a narrow band, which hasa sharp, very definite, but wavy, free margin. It seems to be continuouswith the large spindle-shaped body of the trypanosome, and from thedelicate staining when seen in plan seems to be thin and to becomposed of the same substance as the trypanosome, except at thefree margin, where it takes on the red tint of the micro-nucleus. Thisundulatory membrane, narrowest at the posterior end, where itcommences, gets broader and broader until the micro-nucleus is. R M. Fig. 269.—Blood film from a case of sleeping sickness. Stained byLeishmans method. { x 1000.) a. Blunted end of trypanosome. b. Flagellum. c. Nucleus or macro-nucleus. d. Micro-nucleus or centrosome. e. Undulatory membrane, with red free margin. reached, and then may taper off irregularly as it passes farther forwarduntil it merges into the flagellum. In sleeping sickness the presenceof this organism is usually associated with distinct anaemia, thered cells being diminished in number and the haemoglobin in with this there is an increased number of mononuclear leucocytes,both large and small, in the blood, and cedema and dropsy are commonlypresent. The human subject is said to be an intermediary host of thisorganism, the Glossina palpalis, being the primary host, just as Glossinamorsitans or tsetse fly is the primary host of the trypanosome of I TRYPANOSOMA GAMBIENSE BACTERIA 771 Ngana disease. The try
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