. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 300 J. S. MAKJ ET . .32 .64 . .32 .64 . .32 .64 HEXADECANE (ML) Figure 1. Affinity of mid-exponential phase bacterial cells to hexadecane as a function of hexadecane volume. Results are from three separate batch cultures and are expressed as percentage of the initial absor- bance (A4,Ki) remaining in the aqueous phase as a function of hexadecane volume. A. I'ibrio vulnificus ATCC 27562. B. Isolate DLS1. C. Delcya manna ATCC 25374. D. D. manna ATCC 27129. E. D manna 35142. Bars = standard deviation. mental
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 300 J. S. MAKJ ET . .32 .64 . .32 .64 . .32 .64 HEXADECANE (ML) Figure 1. Affinity of mid-exponential phase bacterial cells to hexadecane as a function of hexadecane volume. Results are from three separate batch cultures and are expressed as percentage of the initial absor- bance (A4,Ki) remaining in the aqueous phase as a function of hexadecane volume. A. I'ibrio vulnificus ATCC 27562. B. Isolate DLS1. C. Delcya manna ATCC 25374. D. D. manna ATCC 27129. E. D manna 35142. Bars = standard deviation. mental data indicate that the bacterial densities of 106 attached cells cm"2 were detectable by B. ncritina larvae. Because the surface free energy of the substratum is such an important factor in the attachment of bryozoan larvae, with larvae attaching in greater numbers to low surface energy, low wettability, hydrophobic surfaces, we used tests to measure both the cell-surface hydrophobic- ity and film wettability of the bacteria to determine if any correlations could be made between these measurements and larval attachment. The results of our cell-surface hy- drophobicity experiments using the adhesion to hexa- decane tests indicated that the three cultures of Deleya marina were the most hydrophobic (had the lowest sur- face free energy) of the five bacteria (Fig. 1). If cell-surface hydrophobicity of the bacteria was the dominant factor favoring bryozoan attachment to surfaces coated with bacteria, the larvae should have attached in greater num- bers to the more hydrophobic (lowest surface free energy) bacteria ( the cultures of D. marina). However, films of D. marina were inhibitory to larval attachment when compared to both unfilmed polystyrene and films com- posed of I'. vulnificus or isolate DLS1. Therefore, it ap- pears that cell-surface hydrophobicity is not the domi- nant factor controlling the attachment of bryozoan lar- vae to surfaces possessing a bacterial fi
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
