. The Biochemical journal. Biochemistry. THE ESTIMATION OF SUGAR IN BLOOD 141 1 minute 40 seconds. This is then controlled by taking 20 cc. of above sodium sulphate solution to which are added 3 cc. of the alkaline copper iodine solu- tion and testing as before. In each case, of course, the adjustment of the flame is brought about by loosening or tightening the screw clip. When a strength of flame is found that brings the mixture to distinct boiling in about 1 minute 40 seconds to 1 minute 45 seconds, the position of the fluid in both limbs of the manometer is marked (see Fig. 1) either by ink
. The Biochemical journal. Biochemistry. THE ESTIMATION OF SUGAR IN BLOOD 141 1 minute 40 seconds. This is then controlled by taking 20 cc. of above sodium sulphate solution to which are added 3 cc. of the alkaline copper iodine solu- tion and testing as before. In each case, of course, the adjustment of the flame is brought about by loosening or tightening the screw clip. When a strength of flame is found that brings the mixture to distinct boiling in about 1 minute 40 seconds to 1 minute 45 seconds, the position of the fluid in both limbs of the manometer is marked (see Fig. 1) either by ink on the glass or on a white card behind, or the difference of level read off and noted. The regu- lating mechanism is now complete, and whenever it is required at a future time all that is necessary is to adjust the gas pressure by means of the screw until the upper surfaces of the coloured liquid stand at the marks indicated. After some time it will be necessary to add more fluid to compensate for slight losses due to Fiff. 1. The gas pressure in this laboratory varies considerably on some days, while on others it remains practically constant for hours. During careful experiments the manometer must be watched and if a change of pressure occurs the necessary adjustment must be made by means of the screw clip. I. Details of Method when 1 cc. of Blood ls used. Coagulation of protein. Exactly 26 cc. of acid sodium sulphate solution are run into a small Erlenmeyer flask from a burette. The flask is fitted with a rubber stopper through which passes a glass tube terminating in a capillary point. Blood is drawn from a vein into a test tube containing a small amount of finely ground potassium oxalate and the tube inverted a few times. One cubic centimetre of the blood is measured in a special pipette made by Hawksley "to contain" 1 cc. The blood is allowed to flow out on to the surface of the fluid in the flask. Any blood adhering to the pipette is then washed o
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