. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HEAT-SHOCK PROTEINS IN MYTILUS 313 sized hsp70 was designated as the response variable (, dependent variable). Because individual mussels were di- vided into separate gill fragments, suhiinils. in the induc- tion experiments, a split-plot ANOVA was used (Sokal and Rohlf. 1981). MONTH and HEIGHT were unit (whole mussel) level effects, and TEMP was a subnnit (gill fragment) level effect. A variable to designate "among whole mussel variation" (MUSS) was also in- cluded in the analysis. In this case, the null hypot
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HEAT-SHOCK PROTEINS IN MYTILUS 313 sized hsp70 was designated as the response variable (, dependent variable). Because individual mussels were di- vided into separate gill fragments, suhiinils. in the induc- tion experiments, a split-plot ANOVA was used (Sokal and Rohlf. 1981). MONTH and HEIGHT were unit (whole mussel) level effects, and TEMP was a subnnit (gill fragment) level effect. A variable to designate "among whole mussel variation" (MUSS) was also in- cluded in the analysis. In this case, the null hypothesis was that the variation in hspVO synthesis was random, and not due to TEMP, MONTH, or HEIGHT. Because the relative level of newly synthesized hsp70 was calcu- lated as a ratio, and because exploratory data analyses revealed unequal variances, these data were log- transformed prior to running the analysis. A similar model was used to analyze the results of the hsp induc- tion experiments with the laboratory-acclimated mus- sels. We were interested in comparing the relative level of newly synthesized hsp70 in relation to incubation temperature and acclimation treatment (pre-acclima- tion (field), 10°, 13°, 17°, and 20°C acclimation). Incuba- tion temperature (TEMP) and acclimation treatment (ACCLTRT) were the independent variables, and the relative level of newly synthesized hsp70 was the depen- dent variable. ANOVA was also used to analyze the results of the hsp70 western blotting. For the field studies, the relative level of endogenous hsp70 was the dependent variable, and collection season, tidal height, and collection time were the independent variables. For the laboratory accli- mation studies, the relative level of endogenous hsp70 was the dependent variable, and acclimation treatment was the independent variable (Sokal and Rohlf, 1981). The Bonferroni correction procedure (Systat) was used for post hoc multiple comparisons (Schlotzhauer and Littell, 1987). Results
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
