. Cytochemistry: a critical approach. Cells. 130 QUANTITATIVE STUDIES will not be resolved, and the transmission of light will be prac- tically nil. If d is of the order of A/3 or A/4, the percentage of transmission is unpredictable. The great danger therefore lies in this region, since the chequers cannot be resolved when d is of this order of A/3 or A/4. Thus, in a specimen in which the ab- sorbing material is aggregated in this way, errors of hundreds of. Fig. 8. To illustrate the absorption of light by a chequerboard distribution of an absorbing substance. The areas containing the absorbin
. Cytochemistry: a critical approach. Cells. 130 QUANTITATIVE STUDIES will not be resolved, and the transmission of light will be prac- tically nil. If d is of the order of A/3 or A/4, the percentage of transmission is unpredictable. The great danger therefore lies in this region, since the chequers cannot be resolved when d is of this order of A/3 or A/4. Thus, in a specimen in which the ab- sorbing material is aggregated in this way, errors of hundreds of. Fig. 8. To illustrate the absorption of light by a chequerboard distribution of an absorbing substance. The areas containing the absorbing substance will be resolvable if d > X/2. percent could arise in calculating the concentration of absorbing substances from extinctions. Probably the most effective way of surmounting this latter difficulty is to study a specimen at several different regions of the spectrum. Errors due to Fixation and Diffusion Displacement of substances in the cell by diffusion may occur at the time of fixation or at a later stage of treatment. As was indicated in Chapter 2, the only reliable method of fixation for tissues is freeze-drying. Diffusion, however, may occur even within the medium used for mounting a section, or during the various stages of a cytochemical reaction. A method of study. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Danielli, J. F. (James Frederic), 1911-. New York Wiley
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