. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 204 MILTON FINGERMAN AND K. RANGA RAO tnonetes, and a mixture of the distilled water extracts from 25 eyestalks of Uca and 25 eyestalks of Palaemonetes were chromatographed on the column of Bio-Gel P-6 (Fig. 3). The above experiments were repeated twice with consistent results. On the basis of these experiments there is no reason to consider the white pig- ment-dispersing substances from the prawn and the crab are anything but identical. In the next experiment the ethanol-soluble fraction of 50 freshly dissected eye- stalks
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 204 MILTON FINGERMAN AND K. RANGA RAO tnonetes, and a mixture of the distilled water extracts from 25 eyestalks of Uca and 25 eyestalks of Palaemonetes were chromatographed on the column of Bio-Gel P-6 (Fig. 3). The above experiments were repeated twice with consistent results. On the basis of these experiments there is no reason to consider the white pig- ment-dispersing substances from the prawn and the crab are anything but identical. In the next experiment the ethanol-soluble fraction of 50 freshly dissected eye- stalks from Uca was prepared taking care to avoid excessive stirring during the extraction. After the alcohol had evaporated the material was eluted in ml distilled water and subjected to chromatography on the Bio-Gel column. The. LOG EYESTALKS PER ML. FIGURE 2. Relationships between the Standard Integrated Response (SIR) of the leuco- phores in Uca pugilator and the logarithm of the relative concentration of extracts of the eyestalks from Palaemonetes vulgar is. See Figure 1 for key to symbols. white pigment-dispersing activity appeared at one place, fraction 7 (Fig. 4). This fraction also had most of the red pigment which is always extracted from the eyestalks along with the chromatophorotropins. The substances in fraction 7 were excluded from this gel which has an exclusion limit of 4600 daltons. No white pigment concentration was observed in this experiment also. Similar results were obtained when a distilled water eluate of the ethanol-soluble fraction from 50 eye- stalks of Palaemonetes was chromatographed (Fig. 4). Previous investigation (Bartell, Rao and Fingerman, 1967) revealed that when aqueous and alcohol extracts of eyestalks from Uca were filtered through Bio-Gel P-6 the melanin-dispersing activity appeared at two peaks, one in the void volume. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability -
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
