. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ) METABOLISM i\ SEA URCHIN EMBRYOS 445. UP C B MB G PR PL FIGURE 3. Incorporation of acctate-1-C14 into total lipids of sea urchin embryos during the early development (solid line). For explanation of dotted line, see text. Abbreviations as in Figure 1. tion of the total lipids as described above. The other portion was centrifuged, washed three times with fre>h sea water and re-suspended in sea water con- taining an excess of C12-acetate. The volume of the final suspension was ad- justed to that of the original susp
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ) METABOLISM i\ SEA URCHIN EMBRYOS 445. UP C B MB G PR PL FIGURE 3. Incorporation of acctate-1-C14 into total lipids of sea urchin embryos during the early development (solid line). For explanation of dotted line, see text. Abbreviations as in Figure 1. tion of the total lipids as described above. The other portion was centrifuged, washed three times with fre>h sea water and re-suspended in sea water con- taining an excess of C12-acetate. The volume of the final suspension was ad- justed to that of the original suspension before the incubation with the isotope, and the suspension was cultured further with constant stirring. \\hen the prism stage was reached after about 24 hours, the embryos were collected and subjected to the extraction procedure. As shown by the dotted line in Fig- ure 3, only a slight decrease in the specific activity of total lipids occurred during the period of development. Since, however, the content of total lipids also somewhat decreased by the prism stage, the slight reduction in the specific activity corresponds to approximately a 20% loss of total radioactivity found in the total lipids of the blastulae. This might lie partly due to the breakdown of lipids which took place after the mcsenchyme blastula stage and partly due to the replacement of C14 by C1- which came from both the culture medium and the metabolic pool. In the same experiment, the total lipids were further separated into an acetone- soluble (non-phospholipids) and an acetone-insoluble (phospholipids) fraction. At the blastula stage, of the radioactivity of total lipids was found in the acetone-soluble fraction and in the acetone-insoluble fraction, while at the prism stage, was recovered from the former fraction and from the latter. If we assume that no interconversion took place between the two fractions. Please note that these images are extracted from scanned page image
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
