. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 70- 80- (0Mm) URN CELL COMPLEX OF SIPUNCULUS NVDVS * * * * 269 (<80/im) DIRECTION OF CELL NOTION •f THIRD TYPE CELLS. (!60-240Mm) M:£@ii/ & FILTERED SEA WATER HEATED SERUM 1 240 /im) and are easily quantifiable with the aid of an ocular micrometer under phase-contrast microscopy. Large arrows indicate direction of UCC swimming motion. Smaller arrows point to third-type cells, which are progressively displaced distalward by the emerging tails. (B) Typical distribution of secretory responses (expressed as percentage of
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 70- 80- (0Mm) URN CELL COMPLEX OF SIPUNCULUS NVDVS * * * * 269 (<80/im) DIRECTION OF CELL NOTION •f THIRD TYPE CELLS. (!60-240Mm) M:£@ii/ & FILTERED SEA WATER HEATED SERUM 1 240 /im) and are easily quantifiable with the aid of an ocular micrometer under phase-contrast microscopy. Large arrows indicate direction of UCC swimming motion. Smaller arrows point to third-type cells, which are progressively displaced distalward by the emerging tails. (B) Typical distribution of secretory responses (expressed as percentage of UCCs with various tail lengths) 20 min after exposure to heated serum (full bars) or filtered seawater (empty bars). electron microscope at an initial accelerating voltage of 75 kV. For scanning electron microscopy, UCCs were pipetted onto round coverslips (20 mm diameter, Carolina Bio- logical Supplies, Burlington, NC) previously coated with poly-D-lysine (Sigma Chemical Co., St. Louis, MO). Cells were then fixed in situ for 60 min at 4°C with a 3% glutaraldehyde buffered with M sodium cacodylate buffer in FSW. After they were rinsed in the same buffer, cells were dehydrated in graded acetones and critical-point dried in a Tousimis device (Tousimis Inc., Rockville, MD) using liquid Freon as transitional fluid. Coverslips were then mounted onto stubs with a thin layer of conductive silver point (Fullam, Schenectady, NY) and sputter-coated with a layer (~ 15 nm) of gold-palladium in a Balzer de- vice (Balzer Union, Nashua, NH). Specimens were viewed in a Jeolco SM-35 scanning electron microscope operated at 10 kV with a 0-60°C stage tilt. Photographs were taken using Polaroid 55 P/N film. Results Effect of serum UCCs exposed to 1:10 heated serum extruded distinct secretory tails as early as 2 min after stimulation (Fig. 1 A). The length of these tails increased after the ensuing 10 min, reaching a peak after 20 min. The average tail length during the observed inter
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
