. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 156 M. A. CURTIN ET AL. was divided into two equal volumes and centrifuged to remove the plasma. One blood pellet was resuspended in buffer to measure iron uptake. The second blood pellet was analyzed for protein content using the Lowry method (Lowry et al., 1951). Protein concentration was taken to be proportional to blood cell concentration. Several metabolic inhibitors such as citric acid (1-9 mA/), 2-deoxyglucose (5 mA/), 4,4-paradinitro-stilbene-2,2 paradisulfonic acid, disodium salt (DNDS) ( mg per ml), and phosphat
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 156 M. A. CURTIN ET AL. was divided into two equal volumes and centrifuged to remove the plasma. One blood pellet was resuspended in buffer to measure iron uptake. The second blood pellet was analyzed for protein content using the Lowry method (Lowry et al., 1951). Protein concentration was taken to be proportional to blood cell concentration. Several metabolic inhibitors such as citric acid (1-9 mA/), 2-deoxyglucose (5 mA/), 4,4-paradinitro-stilbene-2,2 paradisulfonic acid, disodium salt (DNDS) ( mg per ml), and phosphate ( mA/) were studied. The inhibitor was added to both blank and cell suspensions approximately 15 to 30 minutes before the addition of radioactive iron(II). The effect of an inhibitor was also studied by comparing iron uptake in treated and non-treated cell suspensions. Two blood cell suspensions, with approximately the same number of cells, were placed in an ice bath. The first cell suspension was used as a control; the inhibitor was added to the other suspension. The suspensions were incubated for fifteen minutes before adding the iron. The iron remaining in solution was measured as a function of time, for approximately one hour. RESULTS Iron uptake from sea water by S. clava Citrate concentrations up to 5 mA/did not have any visible effect on 5". clava since the rate of oxygen consumption before and after addition of the citrate was the same (Fig. 2). We can therefore assume that there is no acute toxic effect caused by the citrate and that our uptake studies are not affected by the 1000-fold citrate excess used to keep iron(III) from precipitating. cvj O 0. 0. 65 55 CM O E o. 80 75 5mM Citric Acid. Time (h) FIGURE 2. Decline in sea water oxygen over time for (A) a respiration chamber containing 5 adult Slyela clava in liters of jum filtered sea water (12°C) both before and after the addition of citric acid (final concentration approximately 5
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
