. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 224 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS Table I Maintenance schedule for cuttlefish. Sepia officinalis Age (month) Total length (cm) Animal density (cnr/animal) Substrate in tank Food 1 .25 3 no substrate-animals held brine shrimp in sieves with 150 fjm mesh 200 fine sand brine shrimp + grass shrimp ca. 2 training for frozen food 2-4 2-8 500 gravel 5-15 mm mesh size frozen shrimp, squid, fish or clam 4-6 8-12 2000 separation by sex to no substrate frozen shrimp, squid, fish or c


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 224 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS Table I Maintenance schedule for cuttlefish. Sepia officinalis Age (month) Total length (cm) Animal density (cnr/animal) Substrate in tank Food 1 .25 3 no substrate-animals held brine shrimp in sieves with 150 fjm mesh 200 fine sand brine shrimp + grass shrimp ca. 2 training for frozen food 2-4 2-8 500 gravel 5-15 mm mesh size frozen shrimp, squid, fish or clam 4-6 8-12 2000 separation by sex to no substrate frozen shrimp, squid, fish or clam reduce interactions 6+ 12 + 8900 no substrate frozen shrimp, squid, fish or clam ably long, slices. It was not essential to train all animals in a tank during the first week because, if more than 75% of the animals accepted the food within a 24-h period, the other ani- mals in the tank would follow and seek the frozen food. Al- though the cuttlefish would occasionally eat food left in the tank after it was presented, food left for more than 2 h was rejected and had to be removed. Substrate Cuttlefish hatchlings were held in shallow sieves with 150- fjm mesh bottom liners and water flowing in from the top. At the age of 6 weeks, they were transferred to wide shallow tray tanks with a shallow sand substrate. To avoid damage to the skin, the substrate had to present a smooth surface to the cuttle- fish, hence crushed oyster shells were rejected. On the other hand, to reduce bacterial settlement and fouling of the surface, large-grain particles, having a small surface-to-volume ratio, were used in the tanks. When the cuttlefish reached a size of about 2 cm in total length (2 months old), they were transferred to tanks having a thin layer of gravel (grain size of 5-15 mm) as the substrate (Table I). Animal Health Various diseases of cuttlefish in captivity have been described (5). Small numbersot cuttlefish developed severe, focally exten- sive, ulcerative dermatitis and celluli


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology