Induced cell-reproduction and . Fig. 99.—Asymmetrical division induced by decomposed extract. Nostain or atropine is present. AUXETIC IN EXTRACTS 305 unquestionably due to the putrefaction that this onetube of extract induced divisions in the experimentalten minutes. Now, this fact required very careful 100-per-cent solution of extract would not in itselfinduce divisions in lymphocytes unless it was it is fresh this extract is not effective in theexperimental ten minutes. It appeared probable thatthe extract does in itself contain some substancewhich causes


Induced cell-reproduction and . Fig. 99.—Asymmetrical division induced by decomposed extract. Nostain or atropine is present. AUXETIC IN EXTRACTS 305 unquestionably due to the putrefaction that this onetube of extract induced divisions in the experimentalten minutes. Now, this fact required very careful 100-per-cent solution of extract would not in itselfinduce divisions in lymphocytes unless it was it is fresh this extract is not effective in theexperimental ten minutes. It appeared probable thatthe extract does in itself contain some substancewhich causes cell-division, but in the strength of theextract of 100 per cent this substance is not presentin sufficient quantity for it to induce divisions in theexperimental ten minutes unless the whole extract isputrid. The first thing to do was to concentratethe extract and see if this theory was right. It wasappreciated that the concentration process at theroom temperature was a most unsatisfactory pro-cedure, for if the extracts were tightl


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