. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 1. Views of intact and dissected preparations of Sergestes similis- (A) Dorsal view of living intact specimen restrained in testing chamber. Specimen was loosely clamped about the midregion of the body during experimentation. Immediately anterior to the clamp is the hepatopancreas and tbregut. The chamber was superfused with chilled (10°C) filtered seawater. Scale bar = 5 mm. (B) Ventral view of isolated hepa- topancreas showing locations of luminous tissue. Dark pigmentation characteristic of the luminous tissue (arr
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 1. Views of intact and dissected preparations of Sergestes similis- (A) Dorsal view of living intact specimen restrained in testing chamber. Specimen was loosely clamped about the midregion of the body during experimentation. Immediately anterior to the clamp is the hepatopancreas and tbregut. The chamber was superfused with chilled (10°C) filtered seawater. Scale bar = 5 mm. (B) Ventral view of isolated hepa- topancreas showing locations of luminous tissue. Dark pigmentation characteristic of the luminous tissue (arrows) is associated with the (a) anterolateral pair of organs of Pesta. (m) lateral midgastric pair of organs, and (p) posterior fringe organ. Scale bar = 1 mm. an angle of 45°. Typical operating voltage was 34 kV. The anode phosphor of the image intensifier was viewed by a Panasonic newvicon video camera with a 25 mm objective lens, and images were recorded on vid- eotape together with a time and video frame reference. The apparatus for stimulus illumination was as described above. The chamber containing a restrained animal was po- sitioned in the dark in the experimental apparatus. During experiments, the stimulus intensity was either 1 X IfT5 or 2 X 10~4 jiW cm"2. At one-minute intervals, the stim- ulus was briefly extinguished to permit documentation of bioluminescence. Chemical stimulation The physiological basis of the slow photic induction of bioluminescence was further investigated with tests of putative invertebrate neurotransmitters. For this study, specimens of S. similis were collected during the day from the Santa Barbara Basin and thereafter maintained in darkness and handled under dim red light. Intact live an- imals were restrained in the test chamber. In some cases, the hepatopancreas tissue with attached light organs was isolated by dissection, pinned in a clear dish layered with Sylgard, and placed in the test chamber. In some speci- mens, both e
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
