Archive image from page 19 of Current herpetology (2000). Current herpetology currentherpeto2122002niho Year: 2000 ( 78 Current Herpetol 21(2) 2002 among populations by an analysis of covari- ance (ANCOVA). The significance level in all tests was P< Multiple comparisons for means were made following the procedure of the Tukey test (Zar, 1996). Results Estimating initial egg size The eggs of R. t. tagoi used in the experi- ment ranged from to mm (x=, SD=) in diameter at the initial uncleaved stage. Egg diameter increased with the progress of development to mm in
Archive image from page 19 of Current herpetology (2000). Current herpetology currentherpeto2122002niho Year: 2000 ( 78 Current Herpetol 21(2) 2002 among populations by an analysis of covari- ance (ANCOVA). The significance level in all tests was P< Multiple comparisons for means were made following the procedure of the Tukey test (Zar, 1996). Results Estimating initial egg size The eggs of R. t. tagoi used in the experi- ment ranged from to mm (x=, SD=) in diameter at the initial uncleaved stage. Egg diameter increased with the progress of development to mm in mean (SD=) (Fig. 2). The rate of increase was marginal 3 4 5 6 7 8 DEVELOPMENTAL STAGE Fig. 2. The relationship between developmen- tal stages up to the start of gastrulation and ovum diameter (x±SD, n=20) in Rana tagoi tagoi. () up to stage 7, but then became more rapid. The mean diameter of eggs at stage 10 was larger than that at the uncleaved initial stage by . Using the data depicted in Fig. 2, diameters of all eggs measured were con- verted to those at the initial uncleaved stage. Body size and clutch parameters A total of 41 gravid females of R. sakuraii were collected from Bonbori, and 12 and three females were from Takigahara and Kusagyu, respectively. Because the latter two study sites are close to each other (only 5 km apart: Fig. 1) and values for clutch parameters in the three females from Kusagyu were all within the range of corresponding values in the Takigahara females, data for clutch parame- ters from the two sites were pooled as those for a Tochigi population. A total of 34 and 20 females of R. t. tagoi were collected from Ichinosawa and Kami- ange, respectively. We could not determine clutch sizes for parts of these samples, since some females apparently failed to deposit complete clutches. Clutch parameters of females representing the four populations of the two species are shown in Table 1. Clutch size varied from 53 to 186 in R. sakuraii,
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