. A manual of bacteriology. Bacteriology. 26 MANUAL OF BACTERIOLOGY. times. (See below.) The forceps with which it is handled should be sterilized in the flame. Generally bacteria contained in fluids, like sputum, or taken from the surface of a culture, are smeared over the cover-glass by means of the platinum wire or loop, which must be heated to a red heat before and after the operation. Such prepara- FiG. 7.—Cornet forceps for cover-glasses. lions are called smear, cover-glass, cover-slip, or film prep- arations. When the material to be spread is thick or very viscid, a small drop of distil
. A manual of bacteriology. Bacteriology. 26 MANUAL OF BACTERIOLOGY. times. (See below.) The forceps with which it is handled should be sterilized in the flame. Generally bacteria contained in fluids, like sputum, or taken from the surface of a culture, are smeared over the cover-glass by means of the platinum wire or loop, which must be heated to a red heat before and after the operation. Such prepara- FiG. 7.—Cornet forceps for cover-glasses. lions are called smear, cover-glass, cover-slip, or film prep- arations. When the material to be spread is thick or very viscid, a small drop of distilled water must first be placed in the center of the cover-glass so as to dilute it. Beginners generally take too much material on the wire. As thin a smear as possible is made. It is allowed to dry in the air; this should occupy a few seconds. The drying may be hastened by hold-. FiG. 8.—Stewart forceps for cover-glasses. ing the forceps with the cover-glass a long distance above the flame, at a point where the heat would cause no discomfort to the hand. Having dried the preparation, it is to be passed with the smeared surface up three times through the flame of a Bunsen burner or alcohol lamp. This should not be done too slowly and yet sufficiently so to fix the preparation. Various directions are given by different authors as to the time which. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Williams, Herbert Upham, 1866-; Bolton, Benjamin Meade, 1857-1929. Philadelphia, P. Blakiston's Son & Co.
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