. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 102 S. JOHNSEN. Figure 4. Digitized images of arm tips of Aslerias forbesi obtained by overlaying 40 confocal images taken at focal depth intervals of 10 ^m. (A) Control with dark-adapted animal incubated with antibody buffer followed by secondary antibody. (B) Dark-adapted animal incubated with B6-30N antibody fol- lowed by secondary antibody. (C) Light-adapted animal incubated with B6-30N antibody followed by secondary antibody. Brightness and contrast in A, B. and C are identical. Scale bar is 50 /urn. Discussion Biochemi
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 102 S. JOHNSEN. Figure 4. Digitized images of arm tips of Aslerias forbesi obtained by overlaying 40 confocal images taken at focal depth intervals of 10 ^m. (A) Control with dark-adapted animal incubated with antibody buffer followed by secondary antibody. (B) Dark-adapted animal incubated with B6-30N antibody fol- lowed by secondary antibody. (C) Light-adapted animal incubated with B6-30N antibody followed by secondary antibody. Brightness and contrast in A, B. and C are identical. Scale bar is 50 /urn. Discussion Biochemical and immunochemical evidence relating the visual pigment in echinoderms to chore/ale rhodopsins The Western blot analyses and immunohistochemis- try provide four lines of evidence that the protein that cross-reacts with the two monoclonal antibodies is ho- mologous to known rhodopsins. First, the mobility of the protein during SDS-PAGE approximates that of bovine rhodopsin. Second, the protein is insoluble in a physio- logical buffer but soluble in a mild detergent, suggesting that it is membrane-associated. Third, Western blot analysis shows that the purified protein cross-reacts strongly and specifically with two monoclonal antibodies raised against bovine rhodopsin. Finally, both antibodies cross-react with a substance within the optic cups of A. forbesi, and this substance is more abundant in dark- adapted animals than in light-adapted animals. The morphology of the optic cushions and the optic cups of A. forbesi is similar to that found in other Aslerias species that have been studied. Ultrastructural and histofluore- scence work on these congeners has demonstrated the presence of organized microvilli and retinal proteins within the lumen of the optic cups (Eakin and Branden- burger, 1979; Penn and Alexander, 1980; Takasu and Yoshida, 1983). In addition, histotluoresence and ultra-. Please note that these images are extracted from scanned page images that may have been
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
