. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 360 G. GERMAIN AND M. ANCTIL 3 Std. 4. 97,4 66,2 45,0 31,0 21,5 14,4 Immunological revelation with ABC kit Coomassie blue staining Figure 4. Immunoblot analysis of connexin43-like protein in sea pansy membrane homogenates. Lanes 1 and 2 show binding of monoclonal Cx43 antibody to the 43 kDa monomer and the 86 kDa dimer from homogenates of two sea pansies, and lane 3 shows the same from a rat heart homogenate. Lanes 4-6 are Coomassie blue stained blots from the homogenates of lanes 1 -3, presented in the same order. mesogleal
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 360 G. GERMAIN AND M. ANCTIL 3 Std. 4. 97,4 66,2 45,0 31,0 21,5 14,4 Immunological revelation with ABC kit Coomassie blue staining Figure 4. Immunoblot analysis of connexin43-like protein in sea pansy membrane homogenates. Lanes 1 and 2 show binding of monoclonal Cx43 antibody to the 43 kDa monomer and the 86 kDa dimer from homogenates of two sea pansies, and lane 3 shows the same from a rat heart homogenate. Lanes 4-6 are Coomassie blue stained blots from the homogenates of lanes 1 -3, presented in the same order. mesogleal nerve net, a meshwork of neurons and amoe- bocytes embedded in the collagen-based jelly, the meso- glea, that separates ectoderm from endoderm (Fig. 6D). Unlike the luminescent endoderm, in the mesoglea im- munoreactivity was inconsistent, being strong in some locations (Fig. 6D), weak or absent in others. The immu- noreactivity appeared as tiny fluorescent dots distributed in stringlike fashion along tracts of amoebocytes and neurites. The size of the fluorescent dots was in the sub- micrometer range. Lack of resolution made it impossible to determine if the immunoreactivity was associated with either amoebocytes or nerve cells, or both. The pattern of immunoreactivity in the luminescent endoderm was polymorphic. Reactivity usually appeared as punctate immunofluorescence (Fig. 6A), as expected of minute structures the size of gap junctions and as ob- served in tissues examined in this way, including those of the cnidarian Hydra (Fraser et ai, 1987). The fluorescent dots varied in diameter but, as in the mesoglea, all were in the submicrometer range. Owing to the thickness of the sections and the multifocal planes where these small cells are located, the distribution of the reactive dots appeared generally random. Occasionally, however, cells contain- ing refringent granules appeared to be associated with punctate immunoreactivity at their boundaries (Fig. 6A). Less frequently
Size: 1110px × 2252px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
