American journal of physiology . , if in quiet blood, soon beganto contract rhythmically and at the same time to lengthen more witheach relaxation, until a fairly even base line appeared in the writtenrecord. At this stage the addition of fresh quiet blood usually hadno effect, even though the strip were washed once with Ringers Emotional Stimulation of Adrenal Secretion. 67 solution before the second portion of the blood was added. In com-paring the effects of quiet and excited blood on the contracting strip,the two samples were each added to the strip immediately after re-moving the Ringers
American journal of physiology . , if in quiet blood, soon beganto contract rhythmically and at the same time to lengthen more witheach relaxation, until a fairly even base line appeared in the writtenrecord. At this stage the addition of fresh quiet blood usually hadno effect, even though the strip were washed once with Ringers Emotional Stimulation of Adrenal Secretion. 67 solution before the second portion of the blood was added. In com-paring the effects of quiet and excited blood on the contracting strip,the two samples were each added to the strip immediately after re-moving the Ringers solution, orthey were appHed to the strip alter-nately, and the differences in effectthen noted. That blood from the adrenalveins causes the relaxation of intes-tinal muscle characteristic of adre-nal extract is shown in Fig. i. Thestrip was originally beating in bloodwhich contained no demonstrableamount of adrenal secretion; thatblood was replaced by blood fromthe adrenal veins, obtained afterquick etherization. Relaxation oc-. a b c d e Figure 1. — Two fifths the original strip beating in inactive bloodwhich was remov-ed at a. Blood fromadrenal veins substituted at b, and re-moved at c. Contractions restored ininactive blood, removed at d; then bloodfrom renal vein (same animal) added at e. In this and in the other tracings time is recorded in half minutes. curred almost immediately. Then the beats were renewed in the former blood, and thereupon the strip was immersed in blood from the left renal vein, obtained from the same animal and under the same conditions with the adrenal blood. No relaxation occurred. This and other similar tests proved the rehabihty of the method.
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