. Biological structure and function; proceedings. Biochemistry; Cytology. 3l8 SHLOMO HESTRIN Z. Gromet-Elhanan in our laboratory likewise attempted to repeat the result described by Greathouse, and used for this purpose the same strain in conditions which resembled as closely as possible those used by the earlier workers. We have been unable in our laboratory to obtain any significant synthesis of cellulose in this homogenate Fig. 2. Cellulose fibres formed from glucose in a dilute suspension of cells of Acetobacter xyliuiim. Fine filaments (" ultrastrands "), whose thicknes
. Biological structure and function; proceedings. Biochemistry; Cytology. 3l8 SHLOMO HESTRIN Z. Gromet-Elhanan in our laboratory likewise attempted to repeat the result described by Greathouse, and used for this purpose the same strain in conditions which resembled as closely as possible those used by the earlier workers. We have been unable in our laboratory to obtain any significant synthesis of cellulose in this homogenate Fig. 2. Cellulose fibres formed from glucose in a dilute suspension of cells of Acetobacter xyliuiim. Fine filaments (" ultrastrands "), whose thickness as estimated by measurement of shadow length is about 15 A, are seen both in solitary dis- positions and in intertwisted bundles ("composite fibre"). Along the length of some of the latter, aggregations of rods and granules ("amorphous formations") are seen. Photograph was taken at a reaction time of 20 min. Intercellular space at zero time was free from polymer. Specimen is mounted on a collodion film and was freed from non-polymeric solutes by filtration over agar (Kellenberger's technique). Pt shadow-cast (shadow ratio, i :6). Ahignification x 50000. (Elec- tronmicrograph prepared by Dr. D. Danon and Mr. I. Ohad.) The work of Glaser [7] has demonstrated that UDPG labelled in the glucose moiety incorporates ^^C into cellulose in the presence of a sub- cellular particle prepared from A. xylinmn. Mr. I. Ohad in our laboratory has successfully repeated this experiment. However, it should be noted that the cellulose-synthesizing activity manifested by this particle prepara- tion is very poor. It has, moreover, not as yet been shown that the observed. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemist
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