. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. REPORTS FROM MBL GENERAL SCIENTIFIC MEETINGS 347 Evaluation of Spermatocidal Activity of Water Soluble Gossypol on the Sperms of Arbacia punctulata and Spisula solidissima Hiroshi Ueno (Rockefeller University), Tatsuji Haneji, Yumiko Ueno, Samuel S. Koide, and Sheldon J. Segal Gossypol (GP), a phenolic aldehyde isolated from cotton seed, exhibits various biological activities. In vitro effects include in- hibition of sperm motility (1), inactivation of some parasites (2), and inhibition of the infectivity of HIV (3). GP is l
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. REPORTS FROM MBL GENERAL SCIENTIFIC MEETINGS 347 Evaluation of Spermatocidal Activity of Water Soluble Gossypol on the Sperms of Arbacia punctulata and Spisula solidissima Hiroshi Ueno (Rockefeller University), Tatsuji Haneji, Yumiko Ueno, Samuel S. Koide, and Sheldon J. Segal Gossypol (GP), a phenolic aldehyde isolated from cotton seed, exhibits various biological activities. In vitro effects include in- hibition of sperm motility (1), inactivation of some parasites (2), and inhibition of the infectivity of HIV (3). GP is lipophilic and has a maximum attainable concentration in aqueous media of about 100 iiM. Although an effective dose for the in vitro activ- ities is around 1-10 iiM, 10-fold higher doses are required when 5% serum albumin is present in the medium. In some studies, high concentrations of GP are desired to establish dose-response relationships, and this proves difficult because of the limited aqueous solubility. This problem has hampered the development of potentially useful pharmaceutical preparations of GP, such as a protective vaginal cream (4). Water soluble gossypol (GP-S) was prepared at the Institute of Bioorganic Chemistry, Uzbek Academy of Science (Tashkent, USSR). The content of gossypol in GP-S was determined by optical absorption spectrophotometry. The spectrum was ana- lyzed for its shape by log-normal curve fitting analysis, as de- scribed by Metzler et al. (5). GP-S contains gossypol (w/ w), based upon its spectral analysis in 1 TVNaOH and an apparent molar absorptivity of 22,000 cm"1 A/~'. We were able to prepare up to 6 GP-S solution in aqueous media (, M po- tassium phosphate buffer, pH ). Spectra at different pHs (2- 11) showed the identical shift, as observed with the GP standard. Arbacia and Spisula sperms were used, as described before (6). to examine the potency of GP-S to inhibit sperm motility. Figure 1 shows the effect of GP-S on
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